FastQCFastQC Report
Fri 10 May 2024
EGAF00004150462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76310298
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA5953650.7801895885664082No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTAT2422820.31749581163999646TruSeq Adapter, Index 7 (97% over 35bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2126030.278603288903419No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC2075840.27202619494422625No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1996570.2616383440148537No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1771480.23214166979140874No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT1650010.21622376576225663No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1560670.2045163026358513No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1506900.19747007147056353No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1363480.17867575356605214No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT1339320.17550973264447217No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1309920.1716570416223509No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1213320.1589981996925238No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1201390.1574348458185814No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1162180.15229661401662983No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1121650.14698540425041978No Hit
GTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCC1116360.14629218195426258No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1039820.13626208090551553No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1024850.13430035353812927No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT1013070.13275665625103444No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG986370.12925778379216918No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT897680.11763549920876996No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC874440.1145900386865217No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC873860.11451403321737781No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT868600.11382474223859013No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT852800.11175424842398074No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC830370.10881493347070928No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT815970.10692790113334377No Hit
GGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCA788190.1032875012491761No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC771060.10104271903118502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)