FastQCFastQC Report
Thu 16 May 2024
EGAF00004150466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138510043
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA3364620.24291523756151023No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2373140.17133342453730954No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGATATCATCTCGTAT2325360.16788385518008972TruSeq Adapter, Index 10 (97% over 36bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2197140.15862676470326414No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2059910.1487191798792525No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1905390.13756331012040765No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1793300.12947075613859998No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1738780.12553457946728094No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1543600.1114431824990481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)