FastQCFastQC Report
Wed 11 May 2022
EGAF00004150541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38309099
Sequences flagged as poor quality0
Sequence length35-51
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC989620.2583250522284536No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG819190.21383692683558023No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA798010.20830821419214274No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG685960.17905928823854614No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG558100.1456834053967179No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC537180.14022256174701472No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGT513250.1339760039775407No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT473120.12350068582923342No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAG459580.11996627746322094No Hit
TGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGT398890.10412408811807346No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT398280.10396485701738901No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGC396830.10358635686002429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)