FastQCFastQC Report
Wed 1 May 2024
EGAF00004150550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42336292
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT3029670.715620064222913No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC2466700.5826443184962915No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2232660.5273631427145297No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1695950.40059011308784437No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1652690.39037192959647954No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC1557750.36794672523517175No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA1516520.35820803579113636No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC1430020.3377763928876908No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1390110.32834949267640157No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG1363200.32199324399973434No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA1345070.3177108661287578No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG1318380.3114065823242149No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCG1299050.30684075969619634No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1224360.28919868560997264No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1203870.2843588663834802No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1196490.28261568112767177No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1188220.2806622743437238No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1071450.2530807374438933No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA1067790.25221623093491513No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1066320.2518690111075386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1052130.24851727685551678No Hit
CACCTTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGC1034800.24442386215590162No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC1009190.23837467863269649No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1006200.23766842877973346No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC969720.22905170816565606No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC938190.22160419717437702No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC897840.2120733672188391No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG891300.2105285932929601No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC859600.20304092762776674No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG828580.19571388065823056No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA820900.19389983421316162No Hit
CTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTC799930.18894663708385231No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC789220.18641689262725228No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC786580.18579331416175984No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT766380.18102199408488584No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA765000.18069603261428754No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT762400.18008190230736315No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC729350.17227536129049753No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG727840.17191869330455298No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT724180.17105418679557483No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT700190.16538765369437644No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT683590.1614666678886285No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC677840.1601084950944688No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG635160.150027309902341No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA634410.14985015692918974No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG614120.14505757849553758No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCC613980.14502450994054936No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC609610.14399229861698798No Hit
CTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCA597670.1411720232844199No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA576120.13608182785587364No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCA569900.13461263919853916No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG566930.13391111342486017No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT556280.13139554120611224No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG536890.1268155463402416No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG533250.12595576391054747No Hit
CTGCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCC531250.1254833559821441No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC511080.12071912202419616No Hit
CTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACG505830.11947905121213734No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC502730.11874681892311212No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG490140.11577301101381293No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC483200.11413375550225324No Hit
GTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT479820.11333538610325156No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC475680.11235750169145658No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG472130.1115189776185406No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC470180.11105837988834732No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC468790.11073005637810698No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG465430.10993641105838935No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAA460620.10880026999057923No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC451650.10668152043169016No Hit
GTTCGGTTCATCCCGCAGCGCCAGTTCTGCTTACCAAAAGTGGCCCACT445470.10522177993292374No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT445410.10520760769507165No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG442140.10443522073213214No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA439570.10382817654413382No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC437260.10328254538682793No Hit
CTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCG427020.1008638167934027No Hit
CTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGG425080.10040558110285143No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC423950.10013867062330353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)