FastQCFastQC Report
Wed 11 May 2022
EGAF00004150573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35384565
Sequences flagged as poor quality0
Sequence length35-51
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG2043420.5774890831638032No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1760640.49757288241356085No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCG1628640.4602684814692508No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1011550.285873233145582No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG901850.25487101508807586No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT786020.22213640325944375No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA784760.22178031579588442No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT766260.2165520474817198No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT562050.15884044356628377No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGG558750.157907833542676No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG533020.1506363014495162No Hit
CGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG528890.1494691258745162No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA525320.14846021139443144No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG506410.14311607335006096No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG500340.14140063612481882No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG481400.13604801980750647No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG473240.13374192956731276No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGT452960.12801061705859604No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCG440790.12457126433516987No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGT437790.123723437040981No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG436400.1233306103946735No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGG403290.11397342315780906No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG400580.11320755250205845No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG385360.10890624202954029No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCC378130.10686297825054512No Hit
CGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCG362880.10255318950508506No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC359910.10171384048383808No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC358850.10141427483989136No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGA356340.10070492600375333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)