FastQCFastQC Report
Wed 11 May 2022
EGAF00004150649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47500909
Sequences flagged as poor quality0
Sequence length35-51
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATG3696580.7782124758917771No Hit
TGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGT1792180.37729383241908065No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1782140.3751801886570213No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC1720160.36213201730518463No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTA1649970.34735545797660417No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTT1571300.33079366965377444No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTG1528640.32181278888789266No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC1375700.28961551030528704No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAG1300190.27371897240955956No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG1127920.23745229801812848No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG1118620.23549444074849177No Hit
CGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCG1089300.22932192729195983No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCGT1030970.2170421622878838No Hit
CGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCG1008870.21238961974390846No Hit
TTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGG935960.19704043979453112No Hit
GAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTT934480.19672886680968565No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTT904990.19052056456435393No Hit
CGCAGACCCGACGCACCCCCGCCACGCAGTTTTATCCGGTAAAGCGAATG872880.18376069392693095No Hit
AGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGCCCT869060.18295649879037051No Hit
CGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG833390.17544716881102213No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG806900.16987043342686348No Hit
CATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAAT793740.16709996012918407No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA788950.16609155837417766No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG780200.1642494883624227No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA777690.16372107742190786No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGG777330.1636452893985671No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGGG737840.15533176428265827No Hit
GGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG734620.15465388251833243No Hit
CCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCG732620.15423283794421702No Hit
CGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG676850.14249200999500874No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCG648330.13648791436812294No Hit
CTCAGTAACGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCC648010.13642054723626448No Hit
CTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCG639810.13469426448239127No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAT639230.1345721615558978No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT631190.13287956236795384No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC623440.1312480146432566No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCG619730.13046697695827253No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG619450.13040803071789636No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG613670.12919121189870283No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT607280.12784597448440407No Hit
CGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG597640.12581653963716777No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT593610.12496813482032523No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT592730.12478287520771443No Hit
CGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG563470.11862299308840596No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT562610.11844194392153633No Hit
CTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCG561430.11819352762280824No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGAT559820.1178545867406453No Hit
CGGGCGATGGCCTCCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC556640.1171851258678018No Hit
CGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAA551090.11601672717463155No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG548080.11538305509058784No Hit
GTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCG537850.11322941209398751No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA530180.11161470615225491No Hit
CGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA526820.110907351267741No Hit
CACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGT520440.10956421907631284No Hit
ATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAAT519070.10927580354304378No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG518070.10906528125598607No Hit
GTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTC516850.10880844406577567No Hit
CGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCT510630.10749899544027673No Hit
TGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGCCCTCGG509970.10736005073081864No Hit
AAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGG496110.10444221183219884No Hit
CCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTA492670.10371801516472032No Hit
GGAGAAGGGTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTG480080.10106753957066379No Hit
AGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGG477950.10061912709923088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)