FastQCFastQC Report
Thu 16 May 2024
EGAF00004150701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101920298
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT20941682.054711417739379No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG6351890.6232212939565778No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC5147270.5050289393777087No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC4327930.4246386720729565No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA3563110.3495976826912339No Hit
CGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTT3487670.3421958205028011No Hit
CTTGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCT2737390.26858143605506335No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT2356690.23122871952356341No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAA2354480.23101188342286835No Hit
CTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTA2308010.22645243835531173No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC2264390.22217262355335735No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA2174600.2133627984486466No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT2086160.20468542978553692No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA2057100.2018341822352207No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT2053060.20143779406924417No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAA2046010.20074607709643866No Hit
CTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGT2038510.2000102079764327No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAG2002450.19647214924744433No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG1991810.1954281962558626No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA1957080.1920206316508219No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG1944600.19079614543513207No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGAC1937890.19013778786243343No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACT1871120.1835865903767275No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG1842360.18076477759121154No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA1787680.17539980112695508No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1713730.16814413160369684No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA1711710.16794593752070858No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG1635070.16042633627307487No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG1559460.15300779438458864No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT1554420.15251329033594468No Hit
ATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGG1538490.1509503043250521No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCT1529390.15005744979277827No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC1518610.1489997605776231No Hit
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG1410280.13837086700825776No Hit
CACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC1406020.1379528933480944No Hit
CTTGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCT1398470.13721211843395512No Hit
CCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCC1363380.13376923211115416No Hit
CTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACG1355120.13295879492032098No Hit
GTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCG1342920.13176178115177803No Hit
CATGGGTCAGTCGGTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACG1331140.1306059760539554No Hit
CACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGA1330150.13050884133011464No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA1323200.12982693594557582No Hit
CTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACC1317570.12927454352615805No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC1270520.12465819124665431No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC1260310.1236564280846196No Hit
CTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTAATTAAAACAAAG1258690.12349748035469833No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG1213370.1190508685522093No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA1204710.11820118500830913No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC1199210.11766154765363813No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1192800.11703262484573976No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA1188160.11657736715016277No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC1178320.11561190686471502No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAG1177880.11556873587634134No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG1176400.1154235243699935No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1173080.11509777963953755No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC1172190.11501045650396352No Hit
GTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGG1128320.11070611273134229No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC1122870.11017138117080465No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA1117890.10968276407512074No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG1111370.10904304852012894No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAA1092870.10722790469078103No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA1092690.10721024383190088No Hit
TGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGG1089910.10693748167808535No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATC1085910.10654501814741554No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT1083850.10634289942912059No Hit
CCTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG1083810.10633897479381388No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT1077260.10569631576234206No Hit
CAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTG1071700.10515079145471101No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT1062640.10426186155774388No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGG1059580.10396162695678146No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT1059240.10392826755667452No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG1055530.10356425763197827No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC1051000.10311979268349471No Hit
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCC1047080.10273517842343828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)