FastQCFastQC Report
Wed 8 May 2024
EGAF00004150740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23237934
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCTCGTAT11722135.0443942219648275TruSeq Adapter, Index 11 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCGCGTAT4268611.8369145897393462TruSeq Adapter, Index 11 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA727850.31321631260334937No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT578780.2490668920911816No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT564700.24300783365681305No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC518290.22303617868955133No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG419100.18035166121050175No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT392190.16877145791015674No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA371470.15985500260048935No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG367630.15820253211838883No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA272960.11746311010264511No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG244860.10537081308519078No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA240490.10349026725009203No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)