FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004150994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215254
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC499334.108852964071708No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA125861.0356682635893402No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC59980.4935593711273528No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC38810.3193571055927403No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA37770.31079922386595726No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG37590.3093180520286294No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC31530.25945193350525897No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT30580.25163463769713984No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC29820.24538080105064455No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA27290.22456210800375886No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA27030.22242263757206315No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG26970.2219289136262872No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT24260.19962904874207366No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT23760.19551468252727414No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC22540.18547562896316327No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT21600.1777406204793401No Hit
CGCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC21260.17494285145327643No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC20370.16761927959093326No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT20330.16729013029374928No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG20050.16498608521346153No Hit
GCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA17140.14104047384332824No Hit
CGGGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC16700.13741983157430465No Hit
CGAGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC14470.11906975825629869No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG13480.1109233131509956No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA13200.10861926807070785No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG13000.10697352158478804No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG12310.10129569620836466No Hit
AGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG12170.1001436736682208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)