FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004150998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004150998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5382159
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC2294214.262620260754095No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA580161.0779317370594217No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC281270.5225969727018469No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA177080.329012948149618No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG174930.3250182686910587No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC168750.3135358877357581No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC150810.28020353913736107No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC147230.2735519333412484No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT137840.2561054030547964No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA132240.24570065655808385No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA124300.23094821241810212No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG123610.22966619901047144No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT110810.2058839213036999No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT110230.20480628684511176No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC102990.19135443601721913No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT101410.1884188111127895No Hit
CGCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC98500.18301205891539063No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG97180.18055951152687982No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC93460.17364778706834932No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT92170.17125097939321376No Hit
CGGGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC79320.1473758021641501No Hit
GCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA76930.14293520499858886No Hit
CGAGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC70660.13128560490316246No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA63880.1186884296803569No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG60930.11320735786512438No Hit
AACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG59650.11082913009444723No Hit
AGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAG58130.10800498461676811No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG56760.10545953770596521No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG54900.10200367547669996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)