FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004151065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127777
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA223131.978493975316042No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA200331.7763263482053635No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG111950.992660783115811No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA84430.748640910392746No Hit
TTTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG42090.3732120800477399No Hit
GGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA39220.34776378663512375No Hit
GGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA32660.2895962588348583No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG30090.26680806577896166No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA29200.2589164347206939No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG23740.21050260822839975No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG23010.20402969736038243No Hit
CAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCA20440.18124150430448574No Hit
ATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG19700.17467992342457772No Hit
GTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACT19430.1722858331035302No Hit
GAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAA19010.168561692604123No Hit
GGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA18180.16120208161719912No Hit
GGGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA18120.16067006154585525No Hit
CAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCA18030.1598720314388394No Hit
AGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA17640.1564139009751041No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC16680.14790157983360186No Hit
TGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA15800.1400986187872248No Hit
TTGGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG15780.13992127876344349No Hit
GTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG15700.13921191866831828No Hit
GGGAGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA15630.13859122858508377No Hit
GTTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG15570.13805920851373985No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC14520.12874885726522176No Hit
CCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA13840.12271929645665766No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC13240.11739909574321873No Hit
TGGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA13100.11615771557674966No Hit
GGAGTGGGAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA13100.11615771557674966No Hit
TGGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG12970.11500500542217124No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA12960.11491633541028058No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC12620.11190155500599853No Hit
AGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAAC12220.10835475453037258No Hit
TGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA12140.1076453944352474No Hit
TGGGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA11930.10578332418554379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)