FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004151069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733485
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA144121.9648663571852183No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA130061.7731787289446954No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG72310.9858415645855061No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA53240.7258498810473288No Hit
TTTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG26000.35447214326128007No Hit
GGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA25540.34820071303434974No Hit
GGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA21650.29516622698487355No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG21100.28766777780050035No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA16400.2235901211340382No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG14700.20041309638233912No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG13200.17996278042495756No Hit
GTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACT13090.17846309058808293No Hit
GGGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA12260.16714724909166512No Hit
ATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG12170.16592023013422225No Hit
GGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA11860.16169383150303004No Hit
GAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAA11760.16033047710587128No Hit
CAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCA11680.15923979358814427No Hit
AGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA11420.15569507215553147No Hit
GTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG11020.1502416545668964No Hit
CAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCA10920.14887830016973763No Hit
GGGAGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA10320.140698173786785No Hit
TTGGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG9460.1289733259712196No Hit
TGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA9430.12856431965207196No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC9380.12788264245349257No Hit
TGGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG9190.1252922690988909No Hit
GTTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTG9170.12501959821945915No Hit
TGGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA8990.12256556030457337No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA8640.11779381991451768No Hit
GGAGTGGGAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA8280.1128857440847461No Hit
TGGGAGGGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA7800.10634164297838403No Hit
TGGGGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA7560.10306959242520296No Hit
AGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAAC7350.10020654819116956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)