FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004151317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424284
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC249851.0306135749771892No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG77470.3195582695756768No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA68440.28231015838078377No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT59950.24728950898492089No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT49520.20426649682957937No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA33590.13855637375819005No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT32940.13587516974083894No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGG31540.1301002687803904No Hit
GGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTC31000.12787280698136028No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG29150.12024168785505329No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC27860.11492052911292572No Hit
GGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACGAGCGCACGTGTT27830.1147967812352018No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGG25650.10580443545393198No Hit
GGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTC25210.10398946658064813No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)