FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004151425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5908602
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1800883.047895255087413No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA456550.7726870078573578No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG272400.461022759698487No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA218990.37062912682221616No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC218600.369969072210313No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA205840.3483734392670212No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG187970.3181293984600757No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT139690.23641802240191503No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC135320.22902202585315443No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC114420.19364986844603851No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC113780.19256670190342826No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT109200.18481529133287367No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC106680.1805503230713458No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA98430.16658762935801058No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT88760.15022165987825883No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA87640.14832611842869092No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC87610.14827534499700606No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC85020.1438919053948802No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG82910.14032084069971204No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT79060.13380491696682226No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT75890.12843985768545588No Hit
CGCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC74620.12629044907746367No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC73220.12392102226550375No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA71850.1216023688852287No Hit
GTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA71790.12150082202185897No Hit
CAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG70230.1188606035742465No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT69560.11772666359995139No Hit
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA65390.1106691565957565No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT60440.10229154036775535No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA59700.10103912905286225No Hit
AGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA59480.10066679055383998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)