FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004151427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924849
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1802993.0430986511217415No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA464950.7847457378238668No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG278610.4702398322725187No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA221280.3734778726006351No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC217740.36750303678625396No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA211350.3567179517992779No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG177220.2991131081990444No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT142910.24120445938791013No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC142790.24100192258064299No Hit
GGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC116650.1968826547309476No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC114580.19338889480558913No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT111470.18813981588391535No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC106070.17902565955689334No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA101900.17198750550435968No Hit
GTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA88110.14871265073590906No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT87920.14839196745773606No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC87710.14803752804501855No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC84770.14307537626697323No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT81480.1375224921343987No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG81450.1374718579325819No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT78640.13272912102907602No Hit
CGCGGCCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC76580.12925223917098985No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC74290.12538716176564163No Hit
GTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA74250.12531964949655255No Hit
CAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG70200.11848403225128608No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA69580.11743759208040576No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT69480.11726881140768314No Hit
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA67660.11419700316413128No Hit
GGGTTTGGTGGAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG59870.10104898875903841No Hit
CGGGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC59340.10015445119360847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)