FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662169
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC307741.8514362859612954No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA78900.4746809740766432No Hit
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA73500.44219330284706304No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA68520.41223245049089474No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG67330.40507313034956133No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG63330.3810081886980205No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA60300.3627789953969783No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA49770.299428036499297No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC48150.28968173513042295No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG48050.28908011158913444No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT47960.28853865040197474No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC38200.2298201927722151No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA33450.20124307456101034No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC32190.193662617940775No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA31250.18800735665266285No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG30680.18457810246731832No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG30350.18259274478106619No Hit
GATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCA29270.17609521053515015No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT28740.172906605766321No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA28430.1710415727883266No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC27700.16664972093692038No Hit
GCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG26590.1599716996286178No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG25800.15521887365243847No Hit
GGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG24790.1491424758854244No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC23990.14432948755511624No Hit
GGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC23540.1416221816193179No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA23110.13903520039177725No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG22510.13542545914404613No Hit
ACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG21460.12910841196051664No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT21140.12718321662839338No Hit
GACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC21100.12694256721187797No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT20920.12585964483755863No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA20830.12531818365039896No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC20570.12375396244304881No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC20290.12206941652744094No Hit
GGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC19560.11767756467603474No Hit
GGACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC19440.11695561642648854No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT18200.10949548451451085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)