FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055675
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC390391.8990842424021308No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA100010.48650686514162017No Hit
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA93400.4543519768445888No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA87110.4237537548493804No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG84180.40950052902331346No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG77860.3787563695623092No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA77070.3749133496296837No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA62380.3034526372116215No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG61530.29931774234740416No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC60080.29226409816726867No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT60000.2918749315918129No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC48620.2365159862332324No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA43580.21199849197952012No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA39830.1937563087550318No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG39510.19219964245320878No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC39360.19146995512422926No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG37760.18368662361511426No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT36770.17887068724384933No Hit
GATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCA36110.1756600629963394No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA35330.17186568888564582No Hit
GCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG34430.16748756491176864No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC34120.16597954443187762No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG32480.15800162963503472No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC30260.14720225716613763No Hit
GGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG29210.1420944458632809No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA27840.13542996825860118No Hit
GGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC27170.13217069818915927No Hit
GACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC27060.13163559414790763No Hit
ACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG27030.1314896566821117No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG26930.13100319846279204No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT26470.12876549065392145No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC26410.12847361572232965No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT26210.12750069928369026No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA25910.1260413246257312No Hit
GGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC25710.12506840818709183No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC25480.12394955428265655No Hit
GGACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC25090.12205236722730978No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT23400.11383122332080703No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC21290.10356695489316162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)