FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985598
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC375931.8932835347336168No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA96520.4861004090455369No Hit
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA87510.4407236510109297No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA81810.4120169339413114No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG80880.4073332064194263No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG76470.38512327268661634No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA73020.3677481544602684No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA60720.30580208078372356No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG60330.3038379369842234No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC58970.2969886150167355No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT56440.28424686165074703No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC45960.23146679237186982No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA40440.20366660320971314No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC38920.19601147865781493No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA37630.18951469532100657No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG36700.18483096779912148No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG36300.18281646133809562No Hit
GATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCA35830.18044941624639027No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT35700.17979470164655686No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA35500.17878744841604394No Hit
GCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG33470.16856382812633774No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC32420.16327574866614492No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG32010.16121087954359342No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC29280.147461872947092No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA28620.14413793728639937No Hit
GGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG28230.14217379348689915No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG26380.1328567011046546No Hit
GGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC26370.13280633844312897No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC26200.131950173197193No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA25920.1305400186744749No Hit
ACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG25850.13018748004379538No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT25820.13003639205921844No Hit
GACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC25200.12691390704462838No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT24770.12474831259902558No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC24090.12132365161528164No Hit
GGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC23190.11679101207797349No Hit
GGACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC23010.11588448417051186No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT21460.1080782716340367No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC20860.10505651194249793No Hit
AGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG20400.1027398295123182No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)