FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938277
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC363791.8768731197862845No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA93100.4803235038129225No Hit
GGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAA87760.45277326202601587No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA79880.41211859811574925No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG74790.38585816165594494No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG74450.3841040264110857No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA68730.3545932805269835No Hit
TGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGA58750.30310425186905693No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG57020.29417879900550853No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC54940.2834476186840168No Hit
GAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCT53280.27488331131205707No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC44410.22912101830646495No Hit
GGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAA40300.20791661872890202No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA36650.1890854609532074No Hit
TTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGG36260.1870733646429277No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG36040.18593833595507764No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC35790.18464853062797526No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT34440.17768358186162245No Hit
GATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCA33580.1732466515363903No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA33230.171440924078447No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC32320.16674603268779437No Hit
GCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG31600.16303139334573954No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG29020.1497206023700431No Hit
GGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG27860.14373590565228808No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC27840.1436327212261199No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA25920.13372701631397368No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG25470.13140536672518943No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC25340.1307346679550962No Hit
GACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC25090.12944486262799385No Hit
ACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG24860.12825824172705966No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT24590.1268652519737891No Hit
GGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC24290.12531748558126624No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA23920.12340857369715474No Hit
GGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC23620.12186080730463189No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC23500.12124170074762276No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT22700.11711432370089517No Hit
GGACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC21760.11226465567099027No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT20800.10731180321491716No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGAC19900.10266850403734863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)