FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458423
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC101360.6949972675965752No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA80390.551211822633077No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG78850.5406524718823003No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC49480.33927056827820185No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA45180.309786666831228No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG45140.30951239798055846No Hit
GGGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA37990.2604868409233809No Hit
GCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG35690.24471638200988327No Hit
GGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC30860.2115984182915382No Hit
AGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG28760.19719930363138816No Hit
CCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGC27980.1918510610433324No Hit
GGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG27690.18986261187597836No Hit
GGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC26360.18074317259121667No Hit
ACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG26320.18046890374054714No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA25160.17251510707113094No Hit
GACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACC24330.1668240284197383No Hit
GGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG23680.16236715959635853No Hit
GGGCGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGAC23070.15818455962364827No Hit
GTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACG22680.15551043832962042No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA22200.15221921212158612No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG22160.1519449432709166No Hit
TCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGA21270.1458424613435197No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG20310.13926000892745108No Hit
GGACGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC18520.12698647785998987No Hit
CGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGAT18430.12636937294598344No Hit
AGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGG18310.12554656639397485No Hit
GAGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCG16350.11210739271116817No Hit
CGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACG15390.10552494029509958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)