FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004151747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004151747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579133
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC333092.1093220140418825No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA91560.5798118334554467No Hit
GGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGC46690.2956685725648188No Hit
GCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAG45000.28496649743878444No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC42390.26843844058733496No Hit
GATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCA29600.18744462942640044No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTT29450.1864947411016045No Hit
GGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC26860.17009333602679447No Hit
GTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTGCA26840.169966684250155No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA25640.16236757765178741No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC24070.15242541318558983No Hit
GGGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGTG23960.15172882841407279No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC23630.1496390740995217No Hit
TGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA22590.1430531817142698No Hit
GGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGA22340.14147003450627654No Hit
CGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGG21030.13317434313639193No Hit
GGGGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAAGCGGCTGT20700.13108458882184085No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT19440.12310552689355488No Hit
GGAGGGGGGGGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAAG18410.1165829603966227No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT17830.11291005887407836No Hit
GGGAGGGGGGGGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAAA17340.1098070903464116No Hit
GGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGC17220.10904717968657485No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAG17120.10841392080337756No Hit
GGGGAGGGGGGGGTTTGGTGAAAATTTTTTGTTATGATGTCTGTGTGGAA16300.10322119796115971No Hit
CGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGT15980.10119476953492834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)