FastQCFastQC Report
Thu 9 May 2024
EGAF00004167391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29526559
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAAT3271941.1081345442250823No Hit
GTCCTTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCT1202100.4071249887262515No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC904840.30644952566264155No Hit
CTCTGCGGTAAGGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTT904820.3064427520998976No Hit
CTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATG824840.2793552746867659No Hit
CCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGATTGCGGGT792000.2682330846611689No Hit
CTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTAT769690.26067717542027163No Hit
CACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACT747610.25319916215092997No Hit
GTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT699220.23681052709189718No Hit
GTTCGTTTAATCAATCTCCATTGGTTGCTCTTCATCTAATTGAGAATATT691890.23432801634623254No Hit
GTGAGATTAAAGTTAACTACATCTACTTGTGCTATGTAGTTACGAGAATT677360.22940702301273916No Hit
CTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAA629530.2132080477105375No Hit
CCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGT591500.2003281181528806No Hit
CTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAA581240.19685328046522452No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC571890.19368663988241908No Hit
CTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGC551300.1867132570375031No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG521190.17651565832645788No Hit
GTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGA495450.1677980830749699No Hit
CCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCA486130.16464160283628038No Hit
CACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGT482850.16353073854626948No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC478430.16203378117985234No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG468620.1587113486539356No Hit
GTCATTCTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAA468040.15851491533436052No Hit
GGCAATGTTGTTCCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGC446000.15105044919050675No Hit
CTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAA445970.1510402888463908No Hit
CTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAATA445470.15087094977779159No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG440690.149252068281983No Hit
CTCCATTGGTTGCTCTTCATCTAATTGAGAATATTTATTCTCAGTTAGTG433530.14682713281964213No Hit
CCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAATGTTACACAC424690.14383321808680788No Hit
CTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAGG422550.1431084468732032No Hit
CTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAATTTT416110.14092735966964523No Hit
CCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCA406030.13751348404668487No Hit
CTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAG405100.13719851337909034No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG398240.134875181357909No Hit
CCTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTA394660.13366271362673857No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC392840.13304631941703737No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG392390.1328939142552981No Hit
GGCGTGACAAGTTTCATTATGATCTTGCAGTTCAAGTGAGAACCAAAAGA392310.1328668200043222No Hit
CGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTAC390440.13223349188776112No Hit
CTCGTATGTTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTC367850.12458275276844824No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG366300.12405780165579063No Hit
GCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAAT361340.12237795809528634No Hit
GCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAG352130.11925873245168866No Hit
GCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAG351320.11898440316055793No Hit
CACCGGTGATCCAATTTATTCTGTAAACAGCAGCAAGCACAAAACAAGCT350330.11864911180473145No Hit
GCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAATGTTACACA350110.1185746026145478No Hit
AGCACGATTGCAGCATTGTTAGCAGGATTGCGGGTGCCAATGTGATCTTT349530.11837816929497269No Hit
CCTCATAATAATTAGTAATATCTCTGCTATAGTAACCTGAAAGTCAACGA345070.11686766480306765No Hit
CTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCT343380.11629529875120227No Hit
CTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTCTGCCGAAAG339720.11505573676905596No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC339510.11498461436024428No Hit
CTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGTATAATTACCGATAT339260.11489994482594466No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT336460.11395164604178902No Hit
GTGCAAATTTGTTATCAGCTAGAGGATGAAATGGTGAATTGCCCTCGTAT333780.11304398863409718No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG333380.11290851737921781No Hit
CCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGT331710.11234292489009641No Hit
CTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGATTGCGGGTG331500.11227180248128472No Hit
CCTCCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTT330130.11180781343332286No Hit
CTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTC327710.11098821234130263No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG324210.10980283886110806No Hit
CTCCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTA322970.10938287797098199No Hit
AGCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCA320890.10867842744560924No Hit
CCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGC318280.10779447750752127No Hit
GTCCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTC312870.10596222878527768No Hit
CTCCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGG312370.10579288971667847No Hit
CGAGAATTCATTCTGCACAAGAGTAGACTATATATCGTAAACGGAAAAGC311910.10563709777356718No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC311280.10542373054713217No Hit
GTTCCTGGTCCCCAAAATTTCCTTGGGTTTGTTCTGGACCACGTCTGCCG310670.10521713688344111No Hit
CGGTGATCCAATTTATTCTGTAAACAGCAGCAAGCACAAAACAAGCTAAA307870.10426883809928546No Hit
CTCTAACACACTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATC306760.10389290536699518No Hit
TGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAA304520.10313426633967067No Hit
CAGACATTTTGCTCTCAAGCTGGTTCAATCTGTCAAGCAGCAGCAAAGCA298610.10113267854882785No Hit
CTTAGTGACAGTTTGGCCTTGTTGTTGTTGGCCTTTACCAGACATTTTGC297480.10074997225379362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)