FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004167392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29526559
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1126320.3814599594893533No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT788320.2669867491162787No Hit
CTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAG786880.2664990525987129No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT769000.2604434875056047No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC608420.2060585522342783No Hit
CGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTA564470.19117364810440662No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC500570.16953211513742594No Hit
GGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTC486390.16472965915195198No Hit
CCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAAC473660.16041828646541578No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG450390.15253724621280795No Hit
CCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGAT444470.15053227164059316No Hit
CGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACA439770.14894048439576044No Hit
CTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTT425200.1440059439367791No Hit
CGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGAT422710.14316263537515497No Hit
GTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGAT421110.14262075035563745No Hit
CAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTG410490.13902398853858994No Hit
GTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGG405280.13725947544378606No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA378240.1281016186139401No Hit
CTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCC373560.12651660493185135No Hit
CTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATA371780.1259137578476381No Hit
TGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTC352880.11951274105458749No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC349590.1183984899832046No Hit
CCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAA343250.11625127059336647No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG339370.1149371994210365No Hit
CACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAA335640.11367392996928628No Hit
GTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGA330250.11184845480978667No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG329920.11173669102451117No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG323990.10972832967092441No Hit
CTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTT319280.10813315564471972No Hit
CACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATAT314530.1065244344930271No Hit
CTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCT300500.10177278022813292No Hit
GACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGG295950.10023179470387998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)