FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004167393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19713507
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAAT1762390.8940012550785611No Hit
GTCCTTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCT668770.3392445595803933No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC523330.26546773235223947No Hit
CTCTGCGGTAAGGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTT497260.2522432969435626No Hit
CTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATG459500.23308891715715527No Hit
CCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGATTGCGGGT443110.22477482063440057No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC434860.22058987272026231No Hit
CTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTAT431220.2187434229739031No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG430750.2185050077594007No Hit
CACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACT415790.21091630220843No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG396770.2012680950172894No Hit
GTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT389400.19752954154732588No Hit
GTGAGATTAAAGTTAACTACATCTACTTGTGCTATGTAGTTACGAGAATT379310.19241122343173137No Hit
GTTCGTTTAATCAATCTCCATTGGTTGCTCTTCATCTAATTGAGAATATT372820.1891190644059426No Hit
CTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAA359030.18212386055915875No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG342640.17380976403640408No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC330760.16778343904004497No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC330530.16766676776486295No Hit
CTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAA325330.16502898241292124No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG322560.16362385444659847No Hit
CCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGT321000.16283251884101596No Hit
CTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGC313670.15911425602760584No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG306640.1555481731383462No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG294210.1492428516143779No Hit
GTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGA287310.1457427133589168No Hit
CACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGT274400.13919390395630774No Hit
CCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGC266430.13515099063804323No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT263540.13368499070206027No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC261490.13264509455369863No Hit
GTCATTCTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAA257800.1307732814866477No Hit
CCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCA256950.13034210503488802No Hit
GGCAATGTTGTTCCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGC248390.12599990453246093No Hit
CTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAA246340.12496000838409929No Hit
CCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAATGTTACACAC238230.12084607776789791No Hit
CTCATGGATTGTTGCAATTGTTTGGAGAAATCATCCAAATCTGCAGCAGG237130.12028808471267949No Hit
CTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAATA234870.11914166261741252No Hit
CCTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTA233590.1184923616077038No Hit
CTCCATTGGTTGCTCTTCATCTAATTGAGAATATTTATTCTCAGTTAGTG233300.11834525434769166No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG227880.11559587038470628No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC226110.11469800883221844No Hit
CCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCA223260.11325229955278886No Hit
CCCTAATGTTACACACTGATTAAAGATTGCTATGTGAGATTAAAGTTAAC221740.11248125460375975No Hit
CTCGATCGTACTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAG218590.11088336540017968No Hit
CTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAATTTT217480.11032029968082291No Hit
GGCGTGACAAGTTTCATTATGATCTTGCAGTTCAAGTGAGAACCAAAAGA215400.10926518554004623No Hit
CGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTAC214780.10895068036346856No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT212710.10790063888683023No Hit
CTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCT210480.10676943478397831No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT210290.10667305416534967No Hit
GCCTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAG209910.1064802929280924No Hit
CGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC204520.10374612695752206No Hit
GCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAAT202500.10272144880157548No Hit
GCCCATCTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAG200300.10160546269113863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)