FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004167394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19713507
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1072650.5441193188000492No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT652180.3308290097748716No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC568190.28822370367687494No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC473000.23993701374392692No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT460620.2336570555406504No Hit
CTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAG421670.21389902872177946No Hit
CGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTA337900.17140532123482646No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG319410.1620259652430184No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC316730.16066649125394075No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG303560.1539857925837346No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG293560.14891312844538518No Hit
CCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAAC284520.14432744006431733No Hit
GGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTC273930.1389554887418053No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG272830.13839749568658688No Hit
CCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGAT258980.13137185585497294No Hit
CGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGAT248550.1260810671586745No Hit
CGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACA241650.12258092890321341No Hit
GTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGAT234290.11884744809738826No Hit
CAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTG232220.11779740662074992No Hit
GTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGG224970.11411972512044662No Hit
CTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTT222620.11292764904793448No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA217330.11024420971874765No Hit
CTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATA213780.10844341394963361No Hit
CTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCC212450.10776874961923315No Hit
TGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTC204540.10375627228579878No Hit
CGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCG200870.10189460454702454No Hit
CCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAA197310.10008873611377216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)