FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004167395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24068724
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAAT1599300.664472283615866No Hit
GTCCTTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCT584400.24280472865948355No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC579200.24064424852767433No Hit
CTCTGCGGTAAGGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTT541470.22496830326360467No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT532520.22124978457520225No Hit
CTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTAT476670.19804539700567425No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT450500.18717236526539588No Hit
CCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGATTGCGGGT445950.1852819451500628No Hit
CACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACT434990.18072831779532642No Hit
CTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATG412490.17138008645576724No Hit
GTTCGTTTAATCAATCTCCATTGGTTGCTCTTCATCTAATTGAGAATATT404330.16798979455662044No Hit
GTGAGATTAAAGTTAACTACATCTACTTGTGCTATGTAGTTACGAGAATT396440.16471168143354836No Hit
GTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT385100.16000017283841053No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT357670.14860364014311686No Hit
CTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAA334030.13878176508235335No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG320680.13323514782088158No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG320140.13301079026873214No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG314680.13074228613033245No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC314270.13057194058147828No Hit
CCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCA313600.13029357102603362No Hit
CTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAA306460.12732706561428017No Hit
GTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGA300880.1250087042420695No Hit
CCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGT300670.12492145408290029No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA299440.1244104174363377No Hit
CTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGC273870.11378667186511425No Hit
GGCAATGTTGTTCCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGC273240.11352492138760659No Hit
CTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAA270990.11259009825365068No Hit
CTCCATTGGTTGCTCTTCATCTAATTGAGAATATTTATTCTCAGTTAGTG270680.11246130039963896No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG267770.11125226247972264No Hit
GGCGTGACAAGTTTCATTATGATCTTGCAGTTCAAGTGAGAACCAAAAGA259660.1078827444279971No Hit
CTCGTATGTTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTC257560.1070102428363049No Hit
CCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAATGTTACACAC256400.10652828957613208No Hit
CACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGT249910.1038318441808548No Hit
AGCACGATTGCAGCATTGTTAGCAGGATTGCGGGTGCCAATGTGATCTTT244610.10162981635420307No Hit
CTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAATA241030.1001424088788421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)