FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004167397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30267906
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGAAGCTATTAAAATCACATGGGGATAGCACTACTAAAATTAAT2145290.708767233517905No Hit
GTCCTTTTTAGGCTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCT781000.2580290820250334No Hit
CTCTGCGGTAAGGCTTGAGTTTCATCAGCCTTCTTCTTTTTGTCCTTTTT658840.21766950115412678No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC649960.21473570058001368No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT609800.2014675214069979No Hit
CTTGGGTTTGTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTAT561180.18540430249783385No Hit
CTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATG554330.18314117930721735No Hit
CACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACT540470.17856207165437873No Hit
CCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGCAGGATTGCGGGT536560.1772702743295159No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT504680.166737665962092No Hit
GTTCGTTTAATCAATCTCCATTGGTTGCTCTTCATCTAATTGAGAATATT501060.16554167969201436No Hit
GTGAGATTAAAGTTAACTACATCTACTTGTGCTATGTAGTTACGAGAATT483500.15974015513329531No Hit
GTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGCTCATGGATTGTT464240.1533769795637663No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT454820.15026477219798423No Hit
CTGGAGTTGAATTTCTTGAACTGTTGCGACTACGTGATGAGGAACGAGAA448820.1482824745127727No Hit
CCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTGAACAGGAAACTGT414110.13681488240382406No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC408580.13498786470395407No Hit
CTCCGCGTGGCCTCGGTGAAAATGTGGTGGCTCTTTCAAGTCCTCCCTAA393450.1299891707077457No Hit
CCTTGATGTCACAGCGTCCTAGATGGTGTCCAGCAATACGAAGATGTCCA377870.12484180438514644No Hit
CTGCCTTGTGTGGTCTGCATGAGTTTAGGCCTGAGTTGAGTCAGCACTGC367830.12152475959189249No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG364410.1203948499113219No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG360140.11898411472534638No Hit
GTTCTGGACCACGTCTGCCGAAAGCTTGTGTTACATTGTATGCTTTAGTG348190.1150360385023001No Hit
GTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAATGACTTGA345850.1142629424050676No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA336000.11100867037184535No Hit
CTCCATTGGTTGCTCTTCATCTAATTGAGAATATTTATTCTCAGTTAGTG333450.11016619385563046No Hit
CTCTGTTGGTGGGAATGTTTTGTATGCGTCAATATGCTTATTCAGCAAAA330240.10910566459404229No Hit
CACTGATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGT330040.10903958800453524No Hit
GGCAATGTTGTTCCTTGAGGAAGTTGTAGCACGATTGCAGCATTGTTAGC327910.10833587232628515No Hit
CTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATAGAAGTGAATA325360.10749339581007024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)