FastQCFastQC Report
Tue 4 Apr 2023
EGAF00004167406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53522061
Sequences flagged as poor quality0
Sequence length101
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG8224571.5366691503154184No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT6159581.1508488060652224No Hit
CCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGC4184160.7817636170625043No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA2726740.5094609491962576No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC2489010.4650437508376219No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC2192090.40956756130897126No Hit
CCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGC2181550.4075982798943411No Hit
CGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCC2147140.40116915527598984No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC1950240.36438058691349723No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG1943690.36315679248599936No Hit
GTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGG1851720.3459732240131784No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC1670760.31216286682233707No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG1618950.302482746320251No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAAC1562400.29191700969811313No Hit
CTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGT1496220.2795520150092875No Hit
CCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCG1440460.26913388107382485No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG1437520.26858457487278004No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC1424750.26619864283626893No Hit
GGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCC1422700.2658156232062887No Hit
CGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCC1353450.25287703326671224No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1242200.23209121188363804No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT1185880.22156844819559546No Hit
CGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCA1169500.21850802793263135No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1129600.2110531580613086No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1116820.20866535763635857No Hit
CGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCG1105050.20646626444374033No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT1092110.20404856980376745No Hit
GACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCG1091380.20391217744772572No Hit
CGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCG1074780.20081065263910522No Hit
GGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCC1067110.19937759870644742No Hit
CCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCT1030580.19255237573904338No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT1001450.18710976021644607No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA986660.18434641371527152No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCC981550.1833916672229793No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT956450.17870201224127No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCC946780.17689528062082663No Hit
CCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGAC927490.17329115932213449No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG924890.17280537832801318No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC924530.17273811634421177No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT909510.16993179690894192No Hit
CGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGC898770.1679251477255332No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA879580.16433971031123037No Hit
CGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGA872910.16309349522246536No Hit
ACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGG868150.16220414232553565No Hit
CCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAA863900.16141007723899123No Hit
CCCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTC847000.15825250077720288No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG843060.15751635573226525No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC841410.15720807163984213No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT835910.1561804579984317No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC820220.15324895653775367No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG811870.15168885219124878No Hit
GGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGC794760.1484920395722429No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT794750.148490171183804No Hit
CGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGC766460.14320450029007664No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA765770.14307558148779062No Hit
CTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCG757960.14161637011698783No Hit
CCGGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCC751950.14049346866519208No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT750720.14026365688720396No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT728120.13604109901522665No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA722890.1350639318616673No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGG720260.13457254570222923No Hit
GTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCA719950.13451462566062244No Hit
CGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCC716060.13378782255787947No Hit
GGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTC710840.13281252379275904No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT681430.12731759339387172No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG672540.12565659607166474No Hit
GGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCG668600.1249204510267271No Hit
CGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCC665060.12425904151934658No Hit
CGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGA664430.12414133304769412No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG660960.12349300225938607No Hit
GGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGAC656660.12268959523064704No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG649830.1214134859268592No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG635970.11882389955050499No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG632380.11815314810092982No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG631680.11802236091020485No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA630270.117758918140316No Hit
GTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGG623660.1165239133821846No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA621160.1160568162724526No Hit
GGGGGCGGGGCGGACTGTCCCCAGTGCGCCCCGGGCGGGTCGCGCCGTCG607350.113476571838293No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT606170.1132561020024995No Hit
CGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCC598880.11189404683052097No Hit
CCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTG594210.11102150942954157No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT590580.11034328442621072No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC587130.10969869041478056No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTC582220.1087813116912669No Hit
CCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGC580510.10846181726821019No Hit
CCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCT571950.10686247676448782No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG571060.10669619019342323No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA551770.10309206889473109No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT551340.1030117281918572No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT547090.10221766310531277No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG546530.10211303335273281No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA544790.10178793376435934No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC544570.10174682921870293No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA539530.10080516144548318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)