FastQCFastQC Report
Tue 5 Sep 2023
EGAF00004167409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004167409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55613890
Sequences flagged as poor quality0
Sequence length101
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCAC11074511.991320873256663No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG9825671.7667654609307133No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC9036331.6248332925461608No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTT8908821.6019055671164164No Hit
CCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGG7542791.3562780808895045No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC5237370.941737756520898No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC4424100.7955027062483851No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCC4419790.7947277199994461No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG4404010.7918902993478787No Hit
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC3882900.6981888877041329No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC3845470.6914585546884061No Hit
CCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGT3568860.6417209801364372No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC3148010.5660474388682396No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG2983390.5364469200050563No Hit
CCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACC2881000.5180360517849049No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCC2650550.47659856197795186No Hit
CCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTC2484310.44670674897943663No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC2229070.40081173965712524No Hit
CGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTC2200700.3957104960649219No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG2182970.3925224435837881No Hit
CCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCG2144930.3856824257393252No Hit
GCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCC2093140.37637000396843306No Hit
CCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGG2076650.37340491736866455No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG2045940.3678829155809817No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG2039800.3667788748458344No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG1904650.34247739188896875No Hit
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCT1828110.32871464305050413No Hit
CGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGC1776390.31941480806323747No Hit
CCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCC1727620.3106454161001865No Hit
GGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGT1681980.30243883317638814No Hit
GGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCT1655020.29759112336864046No Hit
CCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCT1623930.2920007933269908No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCC1621120.291495523870026No Hit
GCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC1583870.28479755686933605No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG1575710.283330297520997No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG1545030.2778136900691536No Hit
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCG1509100.27135307384540086No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCG1498040.2693643620325785No Hit
CCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACG1447460.2602695118072122No Hit
CCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCG1345490.24193416428881345No Hit
CACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGA1298420.2334704513566665No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG1288890.23175685067165774No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG1288260.23164356961902863No Hit
CCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTC1286500.23132710191644568No Hit
GTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGC1285580.23116167561736825No Hit
CCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGC1220040.21937684992004697No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGC1201640.21606832393849812No Hit
GCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCG1159720.2085306386587955No Hit
CCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTCC1140250.20502971469897177No Hit
CTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGG1135270.2041342549496178No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCC1093210.19657139610266428No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG1077710.1937843225855987No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG1067470.19194305595238886No Hit
AGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCC1057450.19014134778200195No Hit
GGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCG996250.17913690266945903No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCT991110.17821267312896114No Hit
CTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT984320.17699175511729173No Hit
CCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCC981020.17639837817494874No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC956310.17195524355516223No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC954650.17165675697204422No Hit
CGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGA954600.17164776641231175No Hit
CGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC953800.17150391745659221No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT950410.1708943575067308No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCG950120.17084221226028246No Hit
GGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGC942910.16954577354686032No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACC927640.16680005660456407No Hit
GCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCG921000.1656061102720921No Hit
GCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAG915440.16460636002984147No Hit
GGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCG904690.16267338968736048No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA901260.16205663728971306No Hit
CGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCC893700.16069726465816364No Hit
GTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCG880360.1582985833215407No Hit
TGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTC862200.1550332120267077No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG856200.15395434485881135No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCC846740.1522533309574281No Hit
CCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGG843550.1516797332464965No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG840700.15116727134174573No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC839890.15102162427407972No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG819690.14738943814216196No Hit
CGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGGCGAGGGCCGGGGAC801540.14412586495927546No Hit
CCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGA760760.13679316444147316No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGG756420.1360127838566948No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC753200.13543379180992374No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCC750250.13490334878570803No Hit
GGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG749620.13479006773307892No Hit
ACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGAC747330.13437830009733182No Hit
TTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC740950.13323110467546867No Hit
CCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGG735150.13218819974650217No Hit
CTCCTCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCC732960.13179441323021998No Hit
CCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC732310.1316775359536979No Hit
CTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCT731130.1314653587440116No Hit
CTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGT730130.13128554754936222No Hit
GGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTC729990.13126037398211132No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG723310.13005923520185333No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC722600.1299315692536523No Hit
GGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCC720900.1296258902227483No Hit
GGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTT715980.12874121914507328No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT708220.127345884274594No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT703460.12648998298806288No Hit
CACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAGC703160.12643603962966807No Hit
CCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGACGGGGTCCGG691730.12438079767482547No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCT678510.12200369368156047No Hit
GCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCG677420.12180769947939266No Hit
CGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGC676580.12165665807588716No Hit
CTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCC674040.1211999376414777No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG670820.12062094559470665No Hit
GGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGG659090.11851176028146926No Hit
CGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACG658850.1184686055947534No Hit
GGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGA655200.11781229473428312No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG653140.11744188367330535No Hit
CTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCTCTCAGCC652050.11724588947113752No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG650940.11704629904507668No Hit
CCTCTCTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTCCCCGGGGG647110.11635762216956952No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC646700.11628389957976325No Hit
CTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCG631160.11348963361491166No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGC628590.11302751884466272No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT624640.11231726462579761No Hit
CGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGA624350.11226511937934928No Hit
CGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGG618280.11117366542782747No Hit
CCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGG615290.11063602995582578No Hit
GCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCCGGGCCGGGAC611630.109977920983409No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAG611510.10995634364005108No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG606820.10911302913714542No Hit
GGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGA596550.10726636816809615No Hit
CGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGCGGGGAATCCCCCGC594090.10682403262925863No Hit
CGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC591690.10639248576210009No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG585070.10520213565352109No Hit
GCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCC577680.10387333092506205No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTT577100.10376904043216542No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG576780.1037115008498776No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAG576440.10365036504369682No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA567850.10210578688165853No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA567550.1020518435232637No Hit
CTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCC562810.10119953846062558No Hit
CGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGG562310.10110963286330088No Hit
CCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCC559430.10059177662271061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)