FastQCFastQC Report
Fri 31 May 2024
EGAF00004169430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4482959
Total Bases564.8 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG118930.2652935259947726No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG105670.23571484816167176No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC89070.19868573413229967No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT84470.18842465434102787No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTG81290.1813311252679313No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG73790.16460110386911858No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT73380.1636865293659835No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG73010.16286118164364208No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT66370.14804953603189322No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT65360.1457965598168531No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG62500.13941684499010587No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG57110.12739353627815914No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG55810.12449366590236494No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA55780.12442674581676968No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA53940.12032231390026096No Hit
GGGGGCTTTAGCTATTGGCAGCTATGGTGTTCTTAATCCATTTCACATAG53840.12009924694827678No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG52240.11653017571653008No Hit
CTTCATTAAAGTAGTAGCAGTAGGAGCGATAGGCATTGGTGCCTTCTGGG51480.11483486688145039No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT50010.11155578268728311No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC49990.11151116929688629No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC48820.10890128595867149No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC48750.10874513909228258No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT48640.1084997654451No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC48240.1076074976371633No Hit
GCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGA47440.10582296202128996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)