FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004169438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125649
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT85900.40411187359719314No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA70090.32973458929484595No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG68290.3212665872869886No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC66370.3122340518119407No Hit
CCTAGATGTTTCTCCATCCTTTCTTTCTCTGGTTATATTTGAAAAAGAAT45970.21626336238955726No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC44590.20977122751686664No Hit
GCTGGTTGTAAGGGGAGAGATTGTGCTGGTTGTAGAAGCAGAGGTTGTGC43490.2045963374009538No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC38870.18286179891411986No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT37450.1761814862190324No Hit
GTGGTTTCTGAGAAGACACTGTCCTAGATGTTTCTCCATCCTTTCTTTCT37250.17524059710704826No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC37180.1749112859178538No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGT35440.16672555064359168No Hit
CTCTGGTTATATTCGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG32170.1513420136626508No Hit
GCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGTAGGAGCAGAGG31500.1481900351375039No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG31480.14809594622630545No Hit
CTCCATCCTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCA31020.14593190126874192No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGT30880.14527327889035302No Hit
CTTTTTTTCTCTGGCTATATTCAAAACAGAATCTTCCTCGTCACTTGTAG28800.13548803212571783No Hit
CTTTGTTTCTGTGGCCATATTCGGAACAGAATTTTCCTTGTCACTTGTAG28670.13487645420292815No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGC28230.132806498156563No Hit
GGCAGAGGTTGTGCTTGTTGTGGGAGCAGAGGTTGTGCTGGTGGTGGGAA28170.13252423142296776No Hit
CTCTGGTTATATTTGAAAAAGAATCTTTCTCATCACTTGTAGCCTTCAAG28140.13238309805617013No Hit
GTGCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGT26990.12697298566226128No Hit
GCTAGTTGTAGGAGCAGAGGTTGTGCTCGTGGTGGGAACAGGGCTGAGAG26060.12259785129153497No Hit
GCTGGTTGTAGGAACAGAGATTGTGCTGGTTGTAGTAGCTGAGGTTGTGC25350.11925769494399122No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA25120.11817567246520946No Hit
CTCGTCACTTGTAGCCTTCAAGGCTGGTGGTTTCTGAGAAGACACTGTCC25090.11803453909841183No Hit
GGCTTGTGCTGGTCTTTGAAACAGGGGTTGTGCTGGTGGTGGGAACGGGG24320.11441211601727284No Hit
CTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCT24130.1135182713608879No Hit
GGCAGAGGTTGTGCTGCTTATAGGGGCAGAGGTTGTGCTGGTTGTAGGAG23970.11276556007130058No Hit
GTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGG23810.11201284878171326No Hit
CTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGT23340.1098017593685505No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA23220.10923722590136002No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT22200.10443869143024083No Hit
GGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAG22120.10406233578544719No Hit
GTGGGAACAGGGCTGGGAGTACTTCCAGGACCAGAGGTTGTGCTGGCTGT21970.10335666895145906No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT21750.10232169092827649No Hit
GTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCT21490.1010985350826971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)