FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004169439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125649
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT106640.5016820745099497No Hit
CAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACA67180.31604465271547655No Hit
CTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGC59360.27925588843689625No Hit
CAACCTCTGGTCCTGGAACTTCTCTCAGCCCTGTTCCCACCACGAGCACA44310.20845398276008878No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC41960.197398535694275No Hit
CTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT39490.18577855516127073No Hit
GAAAGATTCTTTTTCAAATATAACCAGAGAAAGAAAGGATGGAGAAACAT38330.18032139831176267No Hit
CCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAACT36290.17072432936952434No Hit
CTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCT32950.15501148119938898No Hit
GGAAGATTCTGTTTTGAATATAGCCAGAGAAAAAAAGGATGGAGAAAAAT29200.13736981034968615No Hit
TGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTA29200.13736981034968615No Hit
CAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACCAGCACA27780.13068949765459867No Hit
GAAACATCTAGGACAGTGTCTTCTCAGAAACCACCAGCCTTGAAGGCTAC26770.1259380076390787No Hit
CTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC26570.12499711852709454No Hit
CCAGCCTTGAAGGCTACAAGTGATGAGAAAGATTCTTTTTCGAATATAAC26350.12396214050391198No Hit
CAATCTCTCCCCTTACAACCAGCACAACCTCTGCTCCTATAACCAGCATG26030.12245671792473735No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC25520.12005745068917775No Hit
GCCAGCACAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCAC25330.1191636060327928No Hit
CCAGCACAACCTCTGCTCCTATAACCAGCATGACCTCTGGTCCTGGAACT25020.11770522790921736No Hit
CCAGCACAACCTCTGGTCCTGGAAGTACTCCCAGCCCTGTTCCCACCACC24450.11502369394006254No Hit
CTTCTCGGAAAAAAGCAGCCTTGAAGGCTACAAGTGATGAGAAAGATTCT24420.11488256057326492No Hit
CTTCCACACCACAGACCAGCACAACTTCGGCTCCTACAACCAGCACAACT24360.11460029383966966No Hit
CACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTA24270.11417689373927681No Hit
CCAGCCTTGAAGGCTACAAGTGACGAGGAAGATTCTGTTTTGAATATAGC23810.11201284878171326No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC23700.11149535977012198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23580.11093082630293147No Hit
CCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACC23330.10975471491295129No Hit
CACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAG23160.10895495916776476No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT22840.10744953658859012No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC22740.10697909203259805No Hit
GCACAACCTCTGCTCCTACAACCAGCACAACCTCTGCCTCTACAGCCAGC22280.1048150470750345No Hit
ATCTGCTCCTACAAGCAGCATGACCTCTGGTCCTGGAACTACTCCCAGCC21530.10128671290509392No Hit
ACCAGCACAACCTCTGCCTCTACAGCCAGCACAACCTCTGGTCCTGGAAC21310.10025173488191136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)