FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004169440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076480
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTAGTGGT112040.2748449642829107No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA98050.2405261401993877No Hit
GGGGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTAGTGGTGGT86360.21184943873145456No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT75130.18430116178663947No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA74180.18197071983672186No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG67060.164504670696287No Hit
GTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTAGTGGTGGTGGTGATGGG65910.16168360938849205No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA64910.15923051259910512No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG64390.1579549022686239No Hit
GGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTAGTGGTGGTGG61420.15066920480414475No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG57680.14149462281183767No Hit
GTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTGGTGGTGGTGGTGATGGG56210.1378885705314389No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA55630.13646577439359447No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT54840.1345278279299788No Hit
GGGGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGTGGTGGTGGT54490.13366924405369338No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT52200.12805165240599733No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT51900.12731572336918123No Hit
GTGGGTGTTGGGGTTGGGGTCACCGTGGTGGTGGTGGTGATGGGTGTCGT51420.1261382369102755No Hit
GTTGGGGTTGGGGTCACCGTGGTGGTGGTGGTGATGGGTGTCGTGGTTGG50660.12427388335034147No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG47000.11529554910118534No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA45110.11065919616924406No Hit
GTCGTGGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGTTGGGGTCACCGT44760.10980061229295864No Hit
GTGGAGATGGGTGTCGGGGTTGGGGTCTGTGTGCCGGTGGGTGTTGGGGT44300.10867218776984065No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA43500.10670971033833111No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG43290.10619456001255986No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT42850.10511519742522962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)