FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004169441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4076480
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1059702.599546667713321No Hit
CCCAACCCCAACACCCACCGGCACACAGACCCCAACATCGACACCCATCA106290.2607396577439359No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC68010.16683511264620457No Hit
CACCACCACTACGGTGACCCCAACCCCAACACCCACCGGCACACAGACCC65010.1594758222780438No Hit
CCCACCGGCACACAGACCCCAACATCGACACCCATCACCACCACCACTAC63500.15577164612606956No Hit
CCCCAACCCCAACACCCACCGGCACACAGACCCCAACATCGACACCCATC63490.1557471151581757No Hit
CCCATCACCACCACCACCACGGTGACCCCAACCCCAACACCCACCGGCAC59290.14544410864275062No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA53240.13060287306695975No Hit
CACAGACCCCAACATCGACACCCATCACCACCACCACTACGGTGACCCCA51590.12655526336447132No Hit
CCCCAACACCCACCGGCACACAGACCCCAACATCGACACCCATCACCACC50130.1229737420519664No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT47350.11615413297747076No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC47040.11539367297276082No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT47030.11536914200486693No Hit
CCAACCCCAACACCCACCGGCACACAGACCCCAACATCGACACCCATCAC46200.11333307166967581No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG43640.10705314388884526No Hit
CCCATCACCACCACCACTACGGTGACCCCAACCCCAACACCCACCGGCAC43380.10641533872360467No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC42820.105041604521548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)