FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004169610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004169610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56828792
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4393010.7730254058541312TruSeq Adapter, Index 4 (100% over 50bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG808090.14219728619253424No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA721120.12689342402351259No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)