FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15482542
Sequences flagged as poor quality0
Sequence length37
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG3471202.242009096439073No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2003571.294083361763204No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1880221.2144129820542389No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1839141.1878798714061296No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1823561.1778169243784387No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1758271.1356468466224732No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG1683571.0873989555461887No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1657061.0702764442686479No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1583321.0226486064110145No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG1488560.9614441866200008No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1366860.8828395233805921No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1127670.7283493886210676No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1077070.6956674168880019No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG925720.5979121516350481No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG878020.5671032573333242No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA846660.5468481855240568No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG803510.5189780851232311No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA769290.496875771433399RNA PCR Primer, Index 1 (100% over 37bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT766100.49481538625892313No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA756740.4887698673770754No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT644130.4160363330517689No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT635150.4102362519023039No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG617180.3986296307156796No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG597220.38573769087789334No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC565120.365004661379249No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA535250.34571196383642944No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG534720.34536964278863247No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG488660.3156200060687709No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT476230.3075916086647787No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC466640.30139753536596253No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG456270.29469966882699233No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA454850.2937825067744044No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT452840.29248427034785374No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA440870.2847529817777985No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA434050.2803480203702984No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT424640.2742702070499793No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT419590.271008468764367No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG416300.2688834947129483No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG413260.2669199928538867No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG412300.26629993963523557No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG411110.2655313319996161No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG410730.2652858942672334No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA391410.2528073232418811No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA382880.24729789203865876RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG381080.24613529225368808No Hit
TAAAGATTAAGAGAACCAACACCTCTTTACAGTGACC369280.23851380477443565No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT362070.2338569467468585No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG349900.22599648042291764No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC346130.22356147976217342No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT331030.213808559343808No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA329810.21302057504510563No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT324120.20934546794705933No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG313160.20226652703412656No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT312490.20183378155860968No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC303200.19583347489062194No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG296960.19180312896939017No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC296230.1913316301677076No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT285840.1846208458533489No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG284870.18399433374700355No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC284140.183522834945321No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA283260.1829544528282242No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT280520.181184717599991No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC275780.17812320483290145No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT274880.1775419049404161No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG274730.1774450216250019No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT273620.17672808509093663No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT270760.17488084321037203No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC264460.17081174396297455No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG263730.17034024516129198No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA263600.17025627962126633No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT261160.1686803110238616No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT255920.16529585387205795No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG254980.16468871842879548No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA254230.16420430185172435No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA252980.16339694088993914No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT245920.15883696617777623No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC243460.15724807980498293No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG242720.1567701221156061No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC238590.15410260149786772No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC235320.1519905452218376No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT224430.1449568165227648No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT223710.14449177660877652No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG221050.14277371248209758No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA220300.14228929590502645No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG219200.14157881825865548No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG215410.1391308998225227No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT215380.13911152315943984No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA213500.13789725227291488No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG207870.13426089850103426No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT206590.1334341608761662No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT204480.13207133557267275No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG191130.12344872050080666No Hit
TGAGGTAGTAAGTTGTATTGTTTGGAATTCTCGGGTG189730.1225444762236072No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA189600.12246051068358155No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG187190.12090391874925964No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT185600.11987695560586886No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT184730.11931503237646636No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGAT181400.11716422277427052No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACC181270.11708025723424487No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC178780.11547199419836872No Hit
ATCAATGATGAAACTAGCCAAATCTGAGCTGGAATTC172620.11149331937869117No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG165420.10684292023880834No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGA165080.10662331805720277No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT161690.10443375512884125No Hit
TGTAAACATCCCCGACTGGAAGCTTTGGAATTCTCGG160920.10393642077638156No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT158800.10256713658519383No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA158160.1021537677727598No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA158070.10209563778351126No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT158070.10209563778351126No Hit
GAGAAAGCTCACAAGAACTGCTAACTTGGAATTCTCG155960.10073281248001782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)