FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25746082
Sequences flagged as poor quality0
Sequence length37
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT6622012.5720457194224737No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC3813591.4812312024796628No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT2742321.0651407076230084RNA PCR Primer, Index 2 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG2731821.0610624171864287No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2100790.8159649301202412No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1783400.6926879204377583No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1720410.6682220619044094No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT1578050.6129282117566471RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1534430.5959858280572555No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1489050.5783598451989704No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1430220.5555097664957332No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1421770.552227713715819No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1399000.5433836495976359No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1171400.45498184927710555No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1161990.45132692422870396No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1122360.43593429089521274RNA PCR Primer, Index 1 (100% over 28bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1055720.4100507409243861No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG1038050.40318756073254175No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT999190.3880940020310663No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA878290.34113540071844717RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG852490.33111445850285104No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG842300.32715657473630355No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG826720.3211051685456451No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC814550.3163782357253426RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG814400.31631997443339144No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA813490.3159665225955545No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC798950.310319061362424No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG770940.2994397361120811No Hit
CCCGCCGGGTTGGAATTCTCGGGTGCCAAGGAACTCC719890.27961147641804296RNA PCR Primer, Index 1 (100% over 27bp)
TGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC706560.2744339896066516No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA682140.26494905127700596RNA PCR Primer, Index 1 (100% over 28bp)
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA668740.2597443758627041No Hit
GCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAG655170.25447367098419094No Hit
CCCGCCGGGTCTGGAATTCTCGGGTGCCAAGGAACTC654140.25407361011279306RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA636090.24706283464800585No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG613830.23841685892245662No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG611480.23750409868188876No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG608310.23627284337865467No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT588490.22857458466884398No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG577070.22413895830829716No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG571660.22203766771192604No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT536370.20833072775888775No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG535380.2079462032320102No Hit
CCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGAA534310.20753060601609208RNA PCR Primer, Index 1 (100% over 23bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT525280.2040232762406334No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG515160.20009258107699648No Hit
CGGCTCCGGGACTGGAATTCTCGGGTGCCAAGGAACT511430.19864381695047814RNA PCR Primer, Index 1 (100% over 25bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG490980.19070086081447266No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA485860.18871220871587374No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC484330.18811794353797212RNA PCR Primer, Index 1 (100% over 24bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA483260.18770234632205396No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC482210.187294517278396No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT465910.18096345688637208No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA458380.17803874003042483No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG455920.17708325484242612No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT454770.1766365849374674No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG453660.17620545137702895No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG444800.17276415106578158No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA437630.16997926131051708No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT432410.1679517683506174RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG431100.16744295306757742No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC429000.16662729498026146RNA PCR Primer, Index 1 (100% over 26bp)
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAA422680.16417255254605342RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCA418830.16267717938597415RNA PCR Primer, Index 1 (100% over 28bp)
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC403840.15685493427699018RNA PCR Primer, Index 1 (100% over 26bp)
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC390130.15152985219265594RNA PCR Primer, Index 1 (100% over 27bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG388220.15078799174181143No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAA381960.14835655382438384No Hit
CCGCGAGGGGGGCTGGAATTCTCGGGTGCCAAGGAAC378990.14720298024375125RNA PCR Primer, Index 1 (100% over 24bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC373160.14493855802991695RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT373090.14491136942700641No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA369230.14341211218079705No Hit
CCCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG367450.14272074484964353RNA PCR Primer, Index 1 (100% over 29bp)
ACCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAA361400.14037087274094753RNA PCR Primer, Index 1 (100% over 23bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC353670.13736847416239878RNA PCR Primer, Index 1 (100% over 26bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA351270.13643629349118053No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT348030.1351778495850359No Hit
CCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG344690.1338805648175905RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA343250.13332125641485956No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA342860.1331697770557866No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT335650.13036935095600177No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA333960.1297129404000189No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC331600.128796296073321No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG329650.12803889927795617No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG327670.12726985022420112No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT322230.12515690736943974No Hit
CCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG321030.12469081703383063RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA308890.11997553647191833No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT308810.11994446378287772No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT307770.11954051882534981No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT306210.11893460138905797No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA305650.1187170925657737No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT302340.11743146005671852No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT301930.11727221252538542No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT299230.1162235092702649No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG299140.1161885524950942No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA296510.1151670378428842No Hit
CCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA291810.11334151736174848RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG286420.1112479949376375No Hit
ACTTAGCAGGGCTTGGAATTCTCGGGTGCCAAGGAAC286190.11115866095664576RNA PCR Primer, Index 1 (100% over 24bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA282100.10957006972944466No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC279460.10854467099110458No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG276860.10753480859728483No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG272590.10587630381974236RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTC271650.10551119972351522No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG270430.10503734121564594No Hit
GCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC270200.1049480072346542RNA PCR Primer, Index 1 (100% over 24bp)
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT260650.10123870498043158No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA260430.10115325508556991No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA258690.10047742409893669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)