FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19269407
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG5845403.0335131745361963No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG5765792.9921989815254824No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG4640752.408351227414523No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT4073352.113894838590518No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2294531.1907631615233412No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2045741.0616517674882264No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1951041.01250650837361No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1804170.9362872453729375No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG1426380.7402303558173845No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1333160.6918531535505996No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1260480.6541353348341233No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG1122230.582389484014739No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1113280.5777448159146775No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1082100.5615637263772569No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1060900.550561830989402No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1057030.5485534661237889No Hit
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGG1013000.5257037749008052No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1001210.5195852679846349No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG990880.5142244387697037No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG974750.5058536570430009No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG939020.4873113116558283No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT881460.45744012776314286No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT865420.44911605219610545No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG849180.4406881851631449No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA816940.4239570008563315No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA787100.408471314140596No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT753660.39111738103824367No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT738830.3834212438400414No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG707170.36699105478440514No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG705340.36604136287120825No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG691890.35906138678787575No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG677080.3513756287362657No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC606980.3149967199301982No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG603890.31339314178168537No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA602900.3128793740253657RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA596670.3096462698618593No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG584700.30343435062635815No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC581810.3019345639437685No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA575480.2986495640473005No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT563720.29254662585101865No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG561490.2913893510059754No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT543660.2821363418189257No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATTGGAA541860.2812022186256173No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG532200.27618909082152865No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG525350.2726342331136604No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT520740.2702418398241316No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT508100.2636822191777879No Hit
CCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTC506350.2627740438509602No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT497070.25795811983212563No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA494170.25645314357623983No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC481720.2499921248225231No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG468640.24320416295114838No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG467820.24277861794086344No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG456870.23709603518157046No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA448820.23291842867816326No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG446530.23173001639334312No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA444290.2305675519750037No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG440230.22846058521676355No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTG416870.21633774199693845No Hit
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG411720.2136651117494171No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG407790.21162560944402697No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT399680.20741686550084285No Hit
CCCCACTGCTAAATTTGACTGGCTTTTGGAATTCTCG389570.20217020689842713No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT386900.20078459082835293No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG386140.20039018325784497No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT379820.19711037293467307No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT370810.19243456739483472No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT369300.19165094182711487No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC354250.18384063401639708No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT350400.18184264829737626No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT341550.17724987593027644No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGT335850.17429181915146638No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC333560.1731034068666462No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT327150.16977689038380891No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG323150.1677010610653457RNA PCR Primer, Index 3 (100% over 37bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTG322030.167119828856176No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA316250.1641202554909967No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA313170.16252186691578002No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG312270.1620548053191258No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC306680.15915383384657347No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG306360.15898776750109642No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGA302020.15673549269056386No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG301710.15657461591838295No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT300420.15590516096317858No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG300070.15572352589781305No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG299710.15553670125915137No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT297320.1542963932413696No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG290770.1508972227323861No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG290360.15068445022724364No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG289140.15005132228511237No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA287520.14921061141113476No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC285270.1480429574194992No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCATG279880.14524577741287004No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGA278620.14459189117755414No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT273100.1417272467180749No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC269730.1399783605172697RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG262590.13627300518381288No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTT261490.1357021521212355No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG254530.13209020910710953No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC250440.1299676736289809RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG250080.12978084899031922No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG249570.12951618075221516No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT247930.12866509073164525No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT247710.12855092011912977No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT244960.12712378746268632No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTG234910.12190826630004753No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG226340.11746080198524013No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA221940.11517738973493061No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC216530.11236983058170913No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT214810.11147722397476996No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA213790.11094788749856184No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA211140.10957265057507998No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCCTTG206640.10723734259180887No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA205580.10668724782241612No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC204640.10619942793257728No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG204280.10601260329391557No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT203850.10578945164218079No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC202620.10515113412675335No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG202530.10510442796708792No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTG201870.1047619161295415No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG201290.10446092087836434No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA199800.10368767445723678No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT199420.10349047067198279No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG198700.10311682139465943No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA198240.10287810102303616No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT197440.10246293515934352RNA PCR Primer, Index 1 (97% over 35bp)
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA196770.10211523374850093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)