FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004170901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29240287
Sequences flagged as poor quality0
Sequence length37
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT10779753.6866088215892No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG6008562.054890911296459No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG5109601.7474520684424197No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2634630.9010274078363184No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2360060.8071261407249526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA2283390.780905467856728No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1831570.6263857806867628No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA1827680.6250554243875924No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1736430.5938484803517832No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1630190.5575150476464201No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1421730.48622299774280603No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1154670.39489010487482556No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1100150.3762445970520057RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1072110.36665508789294715No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1071420.36641911209695033No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1011000.3457558402214041No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG910670.31144359150783985No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA839590.2871346645810966RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG835190.28562989138923295No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG828060.2831914748305993No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG827110.28286658061871967No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA826450.2826408646399401No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT821880.2810779524838453No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA764400.26142014269558983RNA PCR Primer, Index 1 (100% over 22bp)
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC762660.2608250732969892No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG734420.2511671653564823No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG732300.25044213827312983No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG722870.24721713572784018No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG716950.24519253179696904No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC685840.23455310134267832No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG671920.22979254615387323No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT640190.21894107947709268No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC602490.2060479091740789RNA PCR Primer, Index 1 (100% over 26bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC563460.19269988697443358RNA PCR Primer, Index 1 (100% over 27bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG558580.19103095670709389No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG541960.18534701796873607RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT540500.18484770686416313RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC524710.17944762306881598No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT521630.1783942818345114No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG515290.17622604046259877No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA507290.1734900892046648No Hit
CCCGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA502130.1717254006432974RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA500590.1711987300261451No Hit
CGGCCGGGCGCGACTGGAATTCTCGGGTGCCAAGGAA497290.17007015013224733RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC489010.1672384405802857No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA484540.16570972781491508No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT470350.16085683427115471No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT455940.15592870206780118No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG446340.15264556055828044No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG445630.1524027448841388No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA444450.15199919207359353No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG443980.15183845493718992No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC442510.15133572389354455No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG439790.150405500465847No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT416580.14246782187876608No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT415890.1422318460827693No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA414640.14180435369871713No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG413040.14125716344713032No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT406110.13888714566994503RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA404390.13829891614948925No Hit
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC401240.13722163534167772RNA PCR Primer, Index 1 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA384620.13153769660331993No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT378010.129277116876452No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG376450.12874360638115487No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT374580.1281040777746128No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC364010.12448920217506758No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG357300.12219442305747547No Hit
AACGAGACTCTGGCATGGAATTCTCGGGTGCCAAGGA356930.12206788531179602RNA PCR Primer, Index 1 (100% over 22bp)
CGGCCCGGACTGGAATTCTCGGGTGCCAAGGAACTCC355250.12149333554762988RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT340590.11647970486746591RNA PCR Primer, Index 1 (100% over 30bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA337830.11553580168347868No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT334950.11455085923062246RNA PCR Primer, Index 1 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGG333530.11406522788233919No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT332510.11371639409695261No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT326010.11149343369988127No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG325860.11144213461379501No Hit
CGGCGCGACCGCTGGAATTCTCGGGTGCCAAGGAACT323800.110737627164877RNA PCR Primer, Index 1 (100% over 25bp)
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA321400.1099168417874968No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC311330.10647296314157245RNA PCR Primer, Index 1 (100% over 27bp)
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG302140.1033300391340208No Hit
ACCCGGCCCGGACTGGAATTCTCGGGTGCCAAGGAAC300540.10278284888243401RNA PCR Primer, Index 1 (100% over 24bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG296110.10126781587335308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)