FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21231674
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG4477322.108792740506472No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2868601.3510945957440754No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG2503051.1789225851904095No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2213231.0424189821301892No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1716950.8086738709345292No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA1679950.7912470773618698No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1440340.6783921041741692No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1415820.6668433209741257No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG1352440.6369916945785811No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1310760.6173606471161907No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1190100.5605304602924857No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1164540.5484918428947242No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1049130.49413437678065325No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG1021160.4809606628285645No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1009370.47540763860635765RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG989280.4659453606908245No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA981680.46236580309211606No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG957900.45116555576352574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG935120.44043630285581814No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA926410.43633394144992993No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT856920.4036045391427921No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG788940.371586338411187No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT782000.3683176371302611No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC730250.3439436758495821No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG714660.33660087282802104No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT696980.3282736914668151No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG658590.31019221564912874No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG658540.3101686659280846No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG650130.3062076028484612No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT635520.2993263743593652No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC632000.29766847399785806No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT622480.29318460711105493No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA621960.2929396900121959No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA620190.29210602988723355No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG604310.2846266384836165No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG603660.28432049211004273No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC590410.27807981603334714No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT562630.2649955910212261No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG544820.25660718038530544No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG535220.2520856339448317No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG531500.2503335346991481No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC527270.24834122829881433No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG518910.24440371494023502No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC493740.23254878536661783No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG493720.23253936547820014No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG480670.22639288828568113No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG468810.22080689445401241No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG464710.21887581732839342No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA464210.2186403201179521No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG459420.216384256841924No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA458110.2157672541505677No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA455350.21446730954893145No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG443380.2088295063309657No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC438210.20639446517500223No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT427760.20147257347677816No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC425490.20040341614137444No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT425440.20037986642033032No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG421450.1985005986810084No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA413040.19453953560138498No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC402320.1894904754095226RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC397860.1873898402923858No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG390940.18413055889987762No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT389630.18351355620852128No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT387400.1824632386499529No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT385080.18137053159350505No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA374810.17653341889103988No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG373990.17614720346591606No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT372120.17526644389886545No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG369010.17380165124992028No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG368980.1737875214172938No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC365730.17225678954942508RNA PCR Primer, Index 5 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG364480.1716680465233217No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG362630.17079670684468873No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA358810.1689975081569169No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT349690.16470203903846675No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT348160.16398141757451626No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA333140.1569070813728583No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT329930.15539518928182489No Hit
GTAGAATTCTCGCCTCCCACGCGGGAGACCCGGGTTT329630.15525389095556008No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG329350.1551220125177129No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG325700.15340288288149112RNA PCR Primer, Index 5 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA321900.1516131040821369No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA305410.1438464060817814No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC300170.14137839531635613No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT296010.13941905852548414No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA295280.13907523259823978No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT293910.1384299702416305No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT291520.13730429357572088No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA290470.13680974943379406No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG286650.1350105507460222No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA285690.13455839610197481No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC284480.13398849285270678No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTTGGAATTC281900.13277332724682941No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG281150.1324200814311674No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT276000.1299944601636216No Hit
TCGGGACTGACCTGAAATGAAGAGAATACTCATTGCT273420.12877929455774423No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT271510.1278796952138583No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC270600.12745109029085505RNA PCR Primer, Index 1 (100% over 27bp)
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT269720.12703661520047832No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA267130.12581673965039214No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA259450.1221995024980131RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG258040.12153540036456852No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC255690.1204285634754942No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG255510.12034378447973533No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG253290.11929817686537576No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA249830.11766853616912167No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC248680.11712689258510658No Hit
TAAAGATTAAGAGAACCAACACCTCTTTACAGTGACC248360.11697617437042411No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC248040.11682545615574165RNA PCR Primer, Index 1 (100% over 27bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT246850.11626497279489124No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGC245000.11539363311625828No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA241410.11370276314528945No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTG239310.1127136748614358No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT238900.1125205671488739No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT238370.11227094010580606No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG234790.11058478007904606No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC233260.10986415861509555RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT231170.10887978027545073No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC230390.10851240462716222No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAA229530.10810734942520311No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT227800.10729252907707608No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG226520.10668965621834622No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG226400.1066331368878403No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG225160.10604910380594577No Hit
TACGGGGATGATTTTACGAACTGAACTCTCTCTTTCT224530.1057523773207897No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG223200.1051259547410157No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT219180.1032325571690673No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT218620.102968800293373No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT213670.1006373779100037No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTC213460.10053846908161833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)