FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18349009
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG8751674.769560034550095No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT7138453.890373589113177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG4225582.3028927611294976No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2200441.1992146278853535No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG2197681.1977104594586008No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT2128121.159801055195951No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG2050501.117499043136335No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA1772710.9661066709379237No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1763860.9612835221782277No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1733910.9449611147937199No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1341580.731145752885074No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG1232550.6717256501427407No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG1225290.6677690331941087No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1181420.6438603850485877No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1119090.6098912480777572No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG990160.5396258729831132No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA879350.4792356906032364No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG702690.3829580115198592No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG698480.380663609680501No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC679680.37041782474464974No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG678240.36963304121764834No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG674150.3674040380055402No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT670120.3652077341070572No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA633740.34538105027906413No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG613910.33457392712598266No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA596120.32487858063615316No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG556050.30304088902021903No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA542700.29576529173864374No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA538410.2934272908144522RNA PCR Primer, Index 1 (100% over 22bp)
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG536630.29245721117690876No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT532880.2904135040753427No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA532810.2903753548761135No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA518350.28249482029247464No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT517770.2821787269274324No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT517120.28182448436316093No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG486560.26516963395679843No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG473070.2578177382767647No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG471050.2567168613847211No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA470090.2561936723667202No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG469260.25574133186157355No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA468030.2550709959322599No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG447550.24390963021490697No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT447040.24363168604909397No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG441830.24079229564931817No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACTGGAA400710.21838236604494551No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTGGAAT399760.21786462691254876No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTGGAA391400.21330852254745747No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT389140.21207684840091365No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCTGG388020.2114664612132459No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA372180.20283384241623076No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATTT351500.19156347898679432No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG336110.1831761050419671No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG331550.18069095720646278No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA330820.18029311555735789RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG328920.17925763729256441No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG327380.1784183549095213No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC313680.17095201163179985No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG312270.170183577761611No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA310790.16937699469219292No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG309330.1685813113939832No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT307200.16742048576029364No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG306140.16684279788625095No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG300970.16402520702889187No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG292970.15966529854555087No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG290970.15857532142471564No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG283780.15465685367531293No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG278690.15188286190278721No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC277110.15102177997732738No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG273070.1488200261932402No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG271240.14782269712767593No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC268550.14635667790015253No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT265370.1446236142780245No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG257840.1405198504180798No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT257100.14011655888337074No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT256820.1399639620864538No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT252300.13750061379336617No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG251450.1370373735170112No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG250730.1366449817535105No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG250410.13647058541417686No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA246080.13411078494756856No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG243320.1326066165208159No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG242060.1319199309346897No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT234780.1279524142148494No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC232760.12685153732280582RNA PCR Primer, Index 1 (100% over 31bp)
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC230410.12557081420582442RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG229380.12500947598859424No Hit
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGG226690.12354345676107086No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT226110.12322736339602863No Hit
GAAAGTCAGCCCTCGACACAAGGGTTTGTGGAATTCT224360.12227363341529779No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT223250.12166869611323423No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC221310.12061141830602405No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG220240.12002828054637718No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT220020.11990838306308531No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT219660.11971218718133499No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG216080.11776112813503987No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG215500.11744503476999765No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA214630.11697089472243433No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG213740.11648585490366264No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA211730.11539042789722323No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG210130.11451844620055501No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG207530.11310147594346921No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAA205070.11176080408484186No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC204910.11167360591517504No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT201890.11002774046271382No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT201420.10977159583931753No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA200280.10915030888044146No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG197650.1077169889665431No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT196380.10702485349481272No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTC194380.10593487637397747No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG193230.1053081395294972No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA192200.10474680131226705RNA PCR Primer, Index 6 (100% over 37bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT189960.10352602693693158No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC188200.10256684707059657No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA187120.10197825942534554No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC185880.10130247361042768No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCT184820.100724785736385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)