FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16844511
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1984341.1780336039437418No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG1719051.020540162905293No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC1593270.9458689539874443No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1529680.9081177838881759No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC1180740.7009642488285946No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1142770.678422781165924No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG1077990.6399651494780704No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT996070.5913320962537886No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG919530.5458929618081522No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG869820.5163818646917088No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA743600.4414494430856437No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT739540.43903916237164736No Hit
AGTGATGATGACCCCAGGTAACTCTTGAGTGTGTCGC722470.42890529739925365No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA716310.42524831976422467No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA699670.4153697308280424No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA685600.4070168614571239RNA PCR Primer, Index 1 (100% over 22bp)
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG682120.4049509065594127No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC674710.40055184742376915No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG572950.3401404766217315No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG565320.33561081114197977No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT548370.32554818599364505No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG545640.32392747999630267No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA518010.3075245105067164No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC510900.30330355093122024No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT480700.2853748618763703No Hit
CTTCGTGATCGATGTGGTGACGTGGAATTCTCGGGTG473930.2813557484690413No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA469940.2789870243190794No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT446500.2650715120195534No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC439160.2607140094479442No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG422380.25075230738369314No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG418370.24837170992972132No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA417580.24790271442133288No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT405090.24048783606719126No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG403210.23937174549026682No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG384050.22799712024884544No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC376680.22362180772122148No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC374020.22204265828791347No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT369850.219567074401863No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA367800.21835006074085497No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG367540.21819570778872713No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC367390.21810665800865336RNA PCR Primer, Index 1 (100% over 26bp)
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG351550.2087030012328645No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC336660.19986332639754278No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG328110.19478748893333858No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT327650.19451440294111239No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATCTAT325360.19315490963198634No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA322240.19130267420645217RNA PCR Primer, Index 7 (100% over 37bp)
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT320840.19047154292576376No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG320120.19004410398140972No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG318190.1888983301444607No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG317830.1886846106722837No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT314930.1869629815908577No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA313260.18597156070603652No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC310940.18459425744089575No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA308240.18299136139956806No Hit
ACTCCATGATGAACACAAAATGACAAGCATATGGCTG305770.18152500835435353No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG304430.18072949698569463No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA302730.1797202661448587No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTG300500.17839639274776214No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG297610.1766807003183411No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT296740.1761642115939133No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTTGGAATTC293580.17428822956035947No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG289280.17173546919824503No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGTGGAATT287800.1708568447015173No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT284340.16880276310781595No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT283850.168511867159575No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG279070.16567414750122458No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA277420.16469459992041324No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG277020.16445713384021654No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG272370.16169659065793004No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGAATTC271650.161269151713576No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG271010.1608892059852613No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA261440.15520783001655553RNA PCR Primer, Index 1 (100% over 23bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC261390.15517814675653094No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG261220.15507722367244736No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA259630.15413329600366552No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT253910.1507375310568529No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC252220.14973423686802187RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT250400.14865376620312695RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC250380.1486418928991171RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG249020.14783450822644836No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC246250.1461900556210863RNA PCR Primer, Index 1 (100% over 31bp)
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTC240400.14271711419820973No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGT239990.14247371146600812No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGTG239570.1422243720818016No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGA238350.1415001005372017No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACACCTCGT237700.1411142181568821No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG232970.13830618175855625No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA232690.13813995550241856No Hit
CTGCGATGATGGCATTTCTTAGGACACCTTTGGATTA232280.13789655277021695No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGGAATTCT232190.1378431229021727No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACTGGAA227590.13511226297991077No Hit
TTCAGATGATGAATTTAACTGTTCAACTGCTGAATGA226080.1342158285271683No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAAT225840.13407334887905026No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTG225120.13364590993469624No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT221900.1317343079891129No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT220430.13086162014439007No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT214450.12731150224544957No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTA214400.12728181898542498No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGA214380.12726994568141517No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG213990.12703841625322337No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT213560.12678314021701192No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTC212240.12599950215236286No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG211950.12582733924422027No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT211950.12582733924422027No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA205290.12187352900894541RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC201870.11984319402326372No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGACCTGGA201040.11935045190685559No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA200850.11923765551876216No Hit
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC198160.11764069612943945No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTG198100.11760507621740993No Hit
TGCCTCTGATGAAGCCTGTGTTGGTAGGGACATCTGA197510.11725481374911982No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACC196860.1168689313688002No Hit
GGTCAATGATGAGTTGGCATGTATTCTGAATCTAAAG195580.1161090399121708No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC191830.11388279541032684No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA191620.11375812571822357No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT190850.11330100351384495No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG190630.11317039716973677No Hit
GCGGCTGATGACAGCACTTCTGCTGAGACGCTGTGAT189420.11245206227714179No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC186140.11050484041952895No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT186000.1104217272914601No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT183420.10889007107419146No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGA180670.10725749177283923No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG180020.1068716093925196No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGAC176700.10490064092688711No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT176550.10481159114681335No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGTGGAATTC174960.1038676634780315No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA172240.10225289413269402RNA PCR Primer, Index 7 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)