FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13230558
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3010012.275043879479611No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2634571.9912765584036591No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT929500.7025402859047971No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG817450.6178499803258487No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG775610.5862262196348786No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA765850.5788493576763731No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA705380.5331445582264935RNA PCR Primer, Index 1 (100% over 23bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC674050.509464529009283No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA606730.45858232132008336No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG560840.42389746524674166No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG552730.41776771622179504No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA538380.4069216128299351No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG523340.39555398948404147No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC512210.38714164587767197No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG510220.385637552097198No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG496780.3754792503838463No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC478750.36185170723713994RNA PCR Primer, Index 1 (100% over 27bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA470840.35587312341626104No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA452380.34192057508080914RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG450770.34070369518806387No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG414790.3135090749762784No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC382890.28939822492747475No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG380490.2875842424786619No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT374270.2828830046321553No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG365180.2760125461072768No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA353710.2673432216539922No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT336500.25433545584396366No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA334760.2530203185685744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA331580.25061679182389734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT331230.25035225271677886No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA325010.24565101487027227No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC289470.21878895810743584RNA PCR Primer, Index 1 (100% over 24bp)
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC259770.19634092530337724RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA256320.1937333255332088No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG253410.19153387181402326No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT248990.18819312080412634RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG247270.1868931000491438No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG240950.1821162796006034No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC239180.18077846754460394No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC237370.17941042244779096No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA236820.17899471813660467No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC229630.1735603290503696RNA PCR Primer, Index 1 (100% over 26bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA227560.17199576918826856No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT225860.1707108649536928No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT223480.16891199902528675No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA223210.16870792599979534No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG220430.1666067296632538No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT217910.16470204809200037No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG215560.16292585694420447No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA210160.15884439643437562No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA209080.15802810433240985RNA PCR Primer, Index 1 (100% over 22bp)
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC207150.15656936011315623RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG204640.15467223680210615No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG204120.15427920727153005No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG201670.1524274335217003No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG196970.14887505122610853No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT195400.14768840437417682No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTT190680.14412090555817827RNA PCR Primer, Index 8 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG188660.14259413699709414No Hit
CCCGGCGGGCGTGGAATTCTCGGGTGCCAAGGAACTC187360.14161156317065388RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG186960.14130923276251842No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT186810.14119585885946762No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG186380.14087085367072197No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC186290.14080282932889152RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG182780.13814987999750275No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT180010.13605624192116464No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT176690.13354689953364024No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC176440.13335794302855555No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC175670.13277595699289477RNA PCR Primer, Index 1 (100% over 24bp)
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT169900.12841484085554064No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG166980.1262078288761517No Hit
CCCCCTCGCGGCCTGGAATTCTCGGGTGCCAAGGAAC165250.1249002498609658RNA PCR Primer, Index 1 (100% over 24bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC158190.11956411815737476No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT157700.11919376340740882No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT153630.11611755150463042No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC153600.11609487672402026No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGA153000.11564138111181704No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC151160.1142506612343939No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT149450.11295819873961477No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA144630.10931511732158235No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG143360.10835521827575224No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG142830.10795463048497275No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT142250.10751625139317632No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT141450.10691159057690537No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA141420.10688891579629521No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA140830.1064429784442954No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC138540.10471213685771984RNA PCR Primer, Index 1 (100% over 26bp)
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG136520.10318536829663572No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG136480.10315513525582216No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC134980.10202139622531416No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG134120.10137138584782289No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCT132580.10020741377650133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)