FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170906

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170906
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17690773
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC2641661.4932417028922367No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG2609071.4748196701184284No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT2112161.1939331311299963No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2092071.18257692866219No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1908311.0787035704997177No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1853761.0478682870443254No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1775961.0038905592197696No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1471620.8318573755934804No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1341460.7582822977831438No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1067050.6031675382415455No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG948190.5359799710278347No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC869550.4915274194067155No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT859700.48595954512558615No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA830670.46954986082292727No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG789890.44649829603262675No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG776420.4388841572948791No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG758750.42889589957431484No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA726440.41063214139936116No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT726050.4104116874938139No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG659620.3728610389155974No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA646990.3657217239744131RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG639240.3613409091846919No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG632900.35775712005348775No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG623750.3525849322694944No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG606730.3429640977248422No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC591500.3343550900800095No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG589070.3329814926685227No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG567150.32059085264391785No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC550520.3111904720048129No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT541740.3062274327978772No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG529830.29949510968231857No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA527720.29830239752666543No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA524490.29647658697559454No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA523360.295837835915932No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG495260.27995384938803974No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG474010.26794193786783654No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA472690.26719578618752277No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT471340.2664326765144745No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA453720.25647268211513424No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA452520.2557943624057581No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT447350.2528719349911957RNA PCR Primer, Index 1 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG433530.24505995300487998No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC424340.23986515456390742No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT416190.23525823320439415No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT405180.22903464987086772No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC399970.2260896117993261No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT399880.22603873782112294No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT398080.22502125825705865No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG380300.21497082122980152No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG373520.21113831487182613No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG370200.20926163034255202No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG365820.20678576340332896No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG365650.20668966811116735No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG357580.2021279680656125No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG357060.20183402952488283No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT339150.19171010786244333No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA332840.18814327672397357No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC319080.1803652107231267No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC309510.17495561104085164RNA PCR Primer, Index 9 (100% over 37bp)
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA304070.17188056169167962No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG300460.1698399498993063No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG294940.16671967923617584No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG294050.1662165921183885No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT293870.16611484416198208No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA289730.16377464116463425No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT286630.16202231524874577No Hit
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC286280.16182447200017772No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC284250.160676981158483RNA PCR Primer, Index 1 (100% over 31bp)
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA283930.16049609590264938RNA PCR Primer, Index 1 (100% over 23bp)
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA281200.15895291856381855No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG275880.15594570118558415No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT271250.15332851764024102No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT269740.15247496533927601No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTC267880.15142356978974295No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA266040.15038347956869946No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC265760.150225204969845No Hit
TCAGATGATGAATTTAACTGTTCAACTGCTGAATGAT264070.14926990471247356No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT262430.1483428677763261No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT262410.14833156244783652No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC256760.14513780714952365No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT254520.14387161035868812No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG252870.14293892075829587No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA249070.1407909083452713No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC248050.14021433659230154RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC247790.1400673673219367No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG247470.13988648206610305No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA246540.1393607842913365No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG238600.1348725688809641No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG236320.13358376143314935No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCA234240.13240800727023064No Hit
GTTCGTTGATGAGGCTCAGAGTGAGCGCTGGGTACAG231920.1310965891654367No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA229520.12973994974668435No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC229100.12950253784840265No Hit
TTCCTATGATGAGGACCTTTTCACAGACCTGTACTGA226400.12797631850230626No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT226190.12785761255316544No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCA226100.1278067385749622No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG225860.127671074633087No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT224950.12715668218681006No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC224780.12706058689464841RNA PCR Primer, Index 1 (100% over 27bp)
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC224180.12672142703996034No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG223450.12630878255008982No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG220790.12480517386097262No Hit
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGA218040.12325069119365219No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTC216600.12243670754240078No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA214680.12135139600739889RNA PCR Primer, Index 7 (97% over 35bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGAC211990.11983082932554728No Hit
TTCCCTTTGTCATCCTATGCCTGTGGAATTCTCGGGT211330.11945775348539038No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT210210.1188246550899726No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG209410.11837244195038849No Hit
TGCAGCTGATGATACAGCTTCTTTCCCCATCAGATCG208720.11798240811749718No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGA207530.11730974107236582No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCT198260.11206972131743481No Hit
GGAGGTGATGAACTGTCTGAGCCTGACCTTGTAGAAT196690.11118225303100096No Hit
TTCTGTGATGAAAATGTTTCTGAAGTGGAATTCTCGG196210.1109109251472505No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGT194220.109786044962535No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG192790.10897771397552836No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC192250.1086724701063091RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG190820.10786413911930248No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA188450.10652445769328452No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA187560.10602137057549718No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG183430.10368682024239416No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG181700.1027089093280435No Hit
CTCCCATGATGTCCAGCACTGGGCTCTGATCACCCCT180050.10177621972765123No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC178420.1008548354557486No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG177500.10033479034522687No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)