FastQCFastQC Report
Fri 31 Mar 2023
EGAF00004170907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27426663
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG8289703.022496757990573No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG6512122.3743756212704406No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT4094011.4927116725793437No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG3953171.4413601829723142No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG3739171.3633339207179525No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG3429291.2503489761040196No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT3273331.1934846029208876No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG3260721.188886887187114No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2771331.0104510344550484No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2286600.8337142582748764No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG2242260.8175475084227345No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT2115300.7712567876011749No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1916600.6988090384892979No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT1716860.6259820963272127No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1632050.5950596323001452No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA1598720.5829072242583795No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAA1439250.5247630745307952No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1388280.5061789689835763No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1320940.481626218982601No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG1190090.43391717030978216No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG1188530.43334838073446996No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG1101930.4017732671306021No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG1077050.3927018026217772No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT1026730.3743546927309385No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG975570.3557013115303163No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGA967690.3528281949575856No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG966270.3523104506005707No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG964750.3517562453733435No Hit
TCGCGTGATGACATTCTCCGGAATCGCTGTACGGCCT946060.3449417087306611No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTTGCT888720.3240350457509176No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG852990.3110075768240562No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA837210.30525405150455237RNA PCR Primer, Index 1 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA830450.3027892966781996No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGAC781920.2850948363641614No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA770110.2807888075920866RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA748770.2730080578887778No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT747100.27239916135623204No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC720860.26283182901251967No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG703170.2563819010719605No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT698900.2548250219138945No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGA693040.25268841491945265No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCT677070.24686561394654535No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGA648820.23656541811156537No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG619310.22580581531191016No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC594790.21686560993584963No Hit
TTTGGCAATGGTAGAACTCACACTGTGGAATTCTCGG587640.21425865771566888No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT582120.21224601767994888No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA569360.20759361064085705No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG562630.20513979407556798No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC561150.2046001731964257No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG541610.19747571915693865No Hit
TGTTGCTGATGCCATCACCGCAGCGCTCTGACCTGGA541540.19745019654779No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC537310.19590790173780892No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG536170.1954922478173885No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA524170.19111694339191027No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG521600.1901798990274537No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG515820.18807246072918168RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG513030.18705520245025797No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC502440.18319399629477345RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT479270.17474601266657924No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA470920.17170153000385063No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG460260.16781480123921746No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC449960.16405933160734867RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC445680.16249880636226144No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG428400.15619836798957276No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG426840.1556295784142606No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG426490.1555019653685175No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT425950.15530507666937096No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT424540.15479097839937728No Hit
CCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTCC400660.1460841225926756RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTC394000.1436558286365352RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG393670.14353550776483454No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGC393010.14329486602143324No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT388980.1418254929518768No Hit
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGG382030.13929146247212065No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACT380690.1388028868112756No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG369990.1349015736985575No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA365020.13308946844900527No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA362570.13219617712880344RNA PCR Primer, Index 1 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAG361780.13190813625412615No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC359840.13120079537200716RNA PCR Primer, Index 10 (100% over 37bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG354240.12915898664011732No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG353550.1289074066356523No Hit
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTG351340.1281016214039601No Hit
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCG347070.12654474224589407No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT344170.12548737700973683RNA PCR Primer, Index 1 (100% over 25bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC339990.12396331263486192RNA PCR Primer, Index 1 (100% over 31bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC335120.122187668255522No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA333370.12154960302680642No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG332980.12140740563297839No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT328530.11978489690853021No Hit
CCCAGGCGGAACTGGAATTCTCGGGTGCCAAGGAACT323680.11801654470323275RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGG323370.11790351600557457No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG319440.11647060380623045No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT318120.11598932031942784No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGG316010.11521999595794792No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTG312300.11386729767307092No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG310950.1133750759252046No Hit
GCAGCTGATGATACAGCTTCTTTCCCCATCAGATCGA308390.11244167764776924No Hit
CGACGGGCGCTGGAATTCTCGGGTGCCAAGGAACTCC306850.11188018024649955RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG304610.11106345675374361No Hit
CAAAGAATTCTCCTTTTGGGCTTTGGAATTCTCGGGT302410.11026131760907261No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGG293950.10717672798911046No Hit
CTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAA292580.1066772140672017No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA291460.10626885232082371RNA PCR Primer, Index 7 (97% over 35bp)
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCTGG288690.1052588862159425No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA287730.10490886186190423No Hit
ATATATGATGACTTAGCTTTTTTCCCCGACAGATCGA286120.10432184185148591No Hit
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGT286060.10429996532935852No Hit
TTGCAGTGATGACTTGCGAATCAAATCTGTCAATCCC284580.1037603444502162No Hit
GGTCCAGGATGAAACCTAATTTGAGTGGACATCCATG283760.10346136531447518No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT278960.1017112435442839No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG275320.1003840678685555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)