FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31565284
Sequences flagged as poor quality0
Sequence length37
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG7803992.472333212652229No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT7731552.449383949784833No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG6985202.2129374790355127No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG6451362.043814970902844No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA3191881.0111995190665797No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG2823860.8946094069674773No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG2390040.7571736088292442No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2142950.6788945729111767No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG2048140.6488584103979549No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG1701440.5390225540185224No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG1565240.4958738847399567No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGA1403370.44459286347621646No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC1237470.39203512314351424No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA1110020.3516584865829181No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1067370.33814680710618666No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA1059730.33572642653872525RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG1059630.3356947461648056No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT1016360.3219866483697723No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG1013580.321105933974806No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG963050.305097841033206No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCTGG960460.304277319348687No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG945640.2995822879337946No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAA943520.29891066400669797No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG935670.29642375465400533No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG921370.29189346118349513No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGC830480.26309916932792365No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA792530.25107646742541584No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG775840.24578901301822598No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG755130.2392280075794661No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG739170.23417181990188968No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT722360.22884634904599624No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAAT711970.22555475819574441No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG707420.22411330118240025No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG706430.2237996654805957No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG702360.22251027426206585No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG688550.21813521462376198No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACTGGAA683580.21656070003995528No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG667560.21148550413802708No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA666890.2112732456327654No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA665010.21067765460307594No Hit
ACTTGCTGTTGAGACTCTGAAATCTGATTTGGAATTC653350.20698372300404455No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA628120.19899076466411642No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC618590.19597162502957363No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT613780.1944477990440384No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG608150.19266419399236198No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGA600290.19017411660227737No Hit
CTTCGTGATCGATGTGGTGACGTGGAATTCTCGGGTG596050.18883086874808414No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG592030.18755731771651413No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT584500.18517178556036437No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGGA582890.18466173154025795No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGA581660.18427206294104626No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGG564050.17869314909379558No Hit
TCTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGC547400.17341836683617357No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT533430.16899261859959822No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG523980.16599882326419113No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG522600.16556163410409994No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT520380.16485832980308368No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC519080.1644464849421282No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG518400.16423105839947458No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC510890.16185186231810872No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG502630.15923506343234547No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCG490160.1552845208045649No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA477290.15120725668110574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT467410.14807723573784415No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA464620.14719335330548586No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC464430.14713316059503853No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA459720.1456410149834229No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG451460.1430242160976597No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC450160.14261237123670423RNA PCR Primer, Index 1 (100% over 26bp)
ATCGATGTGGTGACGTCGTGCTCTCCCGGGTGGAATT447460.14175700114087364No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT428550.1357662424326675RNA PCR Primer, Index 2 (100% over 37bp)
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT427910.1355634880395817No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG423910.13429627308279563No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTGGAA407680.12915454839563617No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGG407600.12912920409650044No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTGGAAT404280.128077415682368No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA402930.1276497306344527No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTTGGAATTC385620.122165857908961No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC383230.12140869697228132RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG376510.11927977584488073No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC372570.11803156911244644No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC369070.11692275602525865RNA PCR Primer, Index 1 (100% over 26bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG368030.11659328013649427No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT362010.11468612162653122No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGTGGAATTC359510.11389411227853993No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT358400.11354246012803179RNA PCR Primer, Index 1 (100% over 25bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCG357650.1133048573236344No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT352050.11153075638413391No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG350210.11094783750401233No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT341820.10828985413215354No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT340390.10783682478510252No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGGCGCTCTGG339720.10762456627984086No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG327960.10389895430688982No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC326720.10350611767028613No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCT324380.10276479692056628No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)