FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18930167
Sequences flagged as poor quality0
Sequence length37
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG12078806.380714972033791No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA5121392.7054119490863444No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT2647731.3986828536694895No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2355701.244415857504057No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG2154251.137998412797943No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG2038221.0767047115854815No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT2002411.0577878156066982No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG1950281.0302497595504572No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG1950061.030133542931766No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG1807330.9547353702690525No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1641530.8671503003644924No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1575910.8324860525530494No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1445970.7638442914951569No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA1345260.7106434930024653No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG1234930.6523608587288215No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1181360.6240621120775109No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1169420.6177547192267242No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA963290.5088650300866338No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG879160.4644227385843981No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG823770.43516256354209665No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG820210.4332819673487297No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG774690.40923569242680213No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA697470.36844365926618605No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC686030.36240039509424293No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC653050.3449784674377146No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT653050.3449784674377146No Hit
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG612510.32356291415707006No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG603410.31875577220211526No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT577070.30484147340063084No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGG568400.30026148210948167No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG553680.29248553380432407No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT548400.28969633495573494No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT531160.28058917810920525No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG514390.27173030222078864No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT513320.2711650668480632No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG509320.26905203741731387No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT503760.26611492650857227No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG499180.26369550781036427No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC488540.25807484952457105No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGG468160.2473089645749031No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG464910.2455921281624193No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA462770.24446165741696838No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG461920.24401263866293413No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG444050.23457267968106146No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA435870.23025153449517904No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG427680.22592510673571978No Hit
CTGGATGATGATAAGCAAATGCTGACTTGGAATTCTC422350.2231094950192463No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT408290.21568219657016235No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC407080.21504300516736063No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT400650.2116463103574311No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG395610.2089838932746869No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG393670.2079590740007735No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG370030.19547107006504483No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT366470.19359047387167794No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGA361620.19102842568689435No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT357260.1887252236073776No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG344150.1817997696480966No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG333170.17599950386068966No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA329760.17419814627097585No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGA321740.16996152226232342No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGG318790.16840316305714578No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG309960.1637386505887666No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT309830.16366997713226722No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT305320.16128753644909735No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTGG283970.15000924186247272No Hit
TCCCTGAGACCCTTTAACCTGTTGGAATTCTCGGGTG283300.1496553094328222No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC278680.14721476044030674RNA PCR Primer, Index 4 (100% over 37bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAAT276500.14606315940054834No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG275550.14556131491074537No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG269940.14259779113411944No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC269200.1422068806894308No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC266680.1408756721480587No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT261660.13822382021246826No Hit
TTCCCTTTGTCATCCTTCGCCTTGGAATTCTCGGGTG260300.1375053902060135No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG255660.13505427606634426No Hit
TGAGAACTGAATTCCATGGGTTGTGGAATTCTCGGGT246820.1303844810243882No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA245600.12974000704800967No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC240120.12684515672788307No Hit
ACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTG237770.12560375193731785No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC237230.12531849296416667No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGTGG236220.12478495303290245No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG232610.12287794397165118No Hit
CCTGGATGATGATAAGCAAATGCTGACTTGGAATTCT229880.12143580138516474No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA227330.12008874512306204No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGA224430.11855679878576876No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTG223970.11831380040123259No Hit
CCTGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA222020.1172836985537423RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATT219470.1159366422916396No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGA214230.11316857373735795No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA213970.11303122682435923RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG212020.11200112497686893No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT210880.11139891158910536No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGATGG210680.11129326011756789No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG210070.11097102312937863No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG208380.11007826819488702No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG207200.10945492451281598No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT198680.10495417182531987No Hit
CCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCG198300.1047534340293987No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA197430.1042938501282107RNA PCR Primer, Index 1 (100% over 22bp)
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC193620.10228118959542194No Hit
CTAGACTGAAGCTCCTTGAGGATGGAATTCTCGGGTG193280.10210158209380826No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA191780.10130919605727726No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)