FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004170911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19385642
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3467481.7886846357732182No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA2514441.2970630531606846No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1878990.9692689053063086No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1372640.7080704368728155No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA1298810.6699855490986576No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1253800.6467673342982398No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG1073950.5539924857789079No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG998980.515319533910716No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG969830.500282631857124No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA945590.48777853217345085No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG928320.4788698769945303No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG895340.4618572859232622No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG839400.4330008776598681No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG821090.4235557429565655No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT789000.40700225455520117No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCTGGAATT758040.3910316717909059No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG621560.3206290511296969No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG608400.3138405217634783No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG566900.2924329253578499No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG565370.2916436814421725No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA561270.2895287140864357No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT525890.2712780933435168No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT520470.2684822096683721No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG507450.2617658986996665No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT506900.2614821835665799No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG505490.260754841134485No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCTGG496550.25614318060758573No Hit
TTTCTGTGTGGAATTTGAATATCTGAAATGGAATTCT494910.255297193665291No Hit
CCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGC439180.22654911299816635No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA432610.2231600067720223RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG432460.22308262991754413No Hit
TATCGGGCCCATGGAATTCTCGGGTGCCAAGGAACTC431270.22246877353868394RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA415830.21450411598439711No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG408040.21048567800849724No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT406520.20970159254978504No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG405240.20904131005823795No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA397760.20518278424825961No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC393630.20305234152162718No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT388040.20016876407807385No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA381030.19655268574546048No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAG363640.18758212908295738No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG352620.1818975095072941No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT346970.1789829813219495No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG340920.17586211485799644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT334260.17242658251916548No Hit
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT332680.17161154631866202RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA332210.17136909884129709No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG330660.17056953801168928No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAA327280.16882597955744774No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA326570.16845972911291768No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACTGGAA323840.1670514703614149No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG323090.16666458608902404No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT315850.16292986324621078No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGA313100.16151128758077757No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT311390.16062919143972637No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG309950.15988637363673588No Hit
ACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCC301500.15552747750113202No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG291990.15062178492721573No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG291620.15043092201950287No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTC291340.15028648522447696No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGA283310.14614424428141198No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC278540.14368366030900603No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA271480.14004178969156658No Hit
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC269050.13878828464902013RNA PCR Primer, Index 1 (100% over 26bp)
CATCATGATGACACGGCTGTGCACAGAGTTGTCTGAC261710.13500197723655477No Hit
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGT259070.13364014459773887No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAAT257740.13295406982136573No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC251710.12984352027134308No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA249100.12849716300342284No Hit
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC248750.12831661700964042RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG243760.1257425469839998No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGT241880.12477275707454001No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT238800.12318395232925482No Hit
GGACAGAAACTGTGTGCTGATTGTCACGTTCTGATTT237680.12260620514915112No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT235290.12137333393446553No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG231580.11945954640037199RNA PCR Primer, Index 5 (100% over 37bp)
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCTG222680.11486851970133359No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT215700.11126791673961585No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCTGGA213980.11038066214159943No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG209420.10802840576546291No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAAT209290.10796134582491516No Hit
CCCGCCGGGCATGGAATTCTCGGGTGCCAAGGAACTC195750.10097679509401855RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)