FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14773903
Sequences flagged as poor quality0
Sequence length37
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG3669642.4838663148120035No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG2999542.030296259559847No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG2560941.7334214256043239No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG1675491.1340875867399427No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT1571341.0635916588866192No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG1445890.9786784169355924No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG1426860.9657975959365647No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG1209550.8187071486796685No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG1144380.7745955824943483No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1134760.7680841007281556No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG1045920.7079510404258104No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT936060.6335901894035719No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG855970.5793797346577949No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT825560.558796142089196No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT751040.5083558488234288No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG742290.5024332432668606No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG720690.4878128684072178No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT717890.48591763462911597No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG708130.47931139117401816No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTG684310.46318836667602326No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT668840.4527172000520106No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA623550.4220617936912135No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA606980.41084607094008946RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACT583550.3949870254326159RNA PCR Primer, Index 1 (100% over 25bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCC580300.3927872005116048No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT578810.39177866539397205No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT554260.3751615263752578No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT552400.373902549651233No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG542790.3673978365771049No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGA530840.3593092495598489No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG492140.3331144112696557No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG487080.32968945308494313No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG484480.32792959314813425No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTG478760.3240579012871548No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG460920.31198255464382024No Hit
CCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAAGGAA443100.299920745384615RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG439540.2975110910095998No Hit
CCCCCCTCGCGGCTGGAATTCTCGGGTGCCAAGGAAC437380.2960490535236356RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA436580.29550755815846363No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG434820.2943162683550853No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG428270.28988277505273996No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA427080.2890773006970467No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTG407710.2759663441678208No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG379700.25700723769473777No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA375230.2539816323418395No Hit
CCCTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCC363140.24579828363567838RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCC354880.240207343990278No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG351870.2381699676788185No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA343540.23253164718896557No Hit
TATTGCACTTGTCCCGGCCTGTTTGGAATTCTCGGGT339380.22971587129007143No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTG338430.2290728455439297No Hit
AGCTACATCTGGCTACTGGGTCTTGGAATTCTCGGGT335830.22731298560712088No Hit
CCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCC330210.22350898066678793RNA PCR Primer, Index 1 (100% over 27bp)
CCGCCGCCGGGCGTGGAATTCTCGGGTGCCAAGGAAC328510.2223583030157975RNA PCR Primer, Index 1 (100% over 24bp)
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC317050.21460138190970932No Hit
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGT308860.20905782310876145No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA305510.2067903112671039No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG303050.20512521301920011No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG302380.20467171065086864No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGT286590.1939839458807872No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG285150.1930092542234777No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG284890.19283326822979682No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGT283590.1919533382613924No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG274140.1855569242602987No Hit
ACGGCCCTGGCGGAGCGCTGGAATTCTCGGGTGCCAA263730.1785107158209987No Hit
CCCGGCGGCGTGGAATTCTCGGGTGCCAAGGAACTCC259130.17539711747125997RNA PCR Primer, Index 1 (100% over 27bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTG251370.17014461242909204No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA251050.16992801428302326RNA PCR Primer, Index 6 (100% over 37bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG245310.16604278503791448No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC244100.16522377329809193No Hit
GCCGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG234030.15840770038898996RNA PCR Primer, Index 1 (100% over 29bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTC226700.15344624910560195No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG224690.15208574200060743No Hit
CCCTGGCGGAGCGTGGAATTCTCGGGTGCCAAGGAAC224000.1516187022481466RNA PCR Primer, Index 1 (100% over 24bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC222030.15028526991141067RNA PCR Primer, Index 1 (100% over 31bp)
TGATGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG217000.14688061780289205No Hit
CGCCGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTC216500.14654218319965956RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGC214530.14520875086292362No Hit
CCGCGAGGGGGGCCCGGGCTGGAATTCTCGGGTGCCA210090.1422034515862193No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT210020.14215607074176675No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTG208770.14130998423368557No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGT208100.14085648186535407No Hit
CCCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCA204380.13833852841730448RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTG203660.13785118258864973No Hit
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAAT196120.13274758877190407No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC194260.1314886120478793RNA PCR Primer, Index 5 (97% over 35bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTG191830.1298438198761695No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACT190460.12891650906331253No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG189040.1279553547901323No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA186410.1261751887771295No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT186310.126107501856483No Hit
CCCCGGGGAGCCCGGCGGGCTGGAATTCTCGGGTGCC183390.12413104377360538No Hit
TAAAGTGCTTATAGTGCAGGTAGTGGAATTCTCGGGT176240.11929142894738105No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTTGGAATTCT174590.11817459475671392No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA174000.1177752419248996No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG173070.11714575356288721No Hit
CCCTGGCGGAGCTGGAATTCTCGGGTGCCAAGGAACT171490.1160763002166726RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCGCGGACTGGAATTCTCGGGTGCCAAGGAACTC171290.1159409263753796RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG171080.11579878384202198No Hit
TGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGG169870.1149797721021994Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCA165680.1121436901271113No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG162920.11027553111726807No Hit
CATTAGGAGGGCTGAGAGGGCCCCTGTAATGGAATTC162170.10976787921241936No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC159850.1081975426534207No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG156570.10597741165621569No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGG155050.10494857046238898No Hit
TCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTG153490.10389265450030367No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGG152330.10310748622080436No Hit
CCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA152050.10291796284299416No Hit
GCGGGCCGCCGGTGAAATACCACTACTCTGATGGAAT151490.1025389160873738No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTC149310.10106334121728024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)