FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004170913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004170913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18129695
Sequences flagged as poor quality0
Sequence length37
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGG17755729.793722398529043No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGG11032866.085518813195699No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGT7677904.234985751277118No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGG5306822.9271424588223907No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTG3566571.9672531722127702No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTG3187191.7579942740349468No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAA2996731.6529401073763237No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTG2155051.1886851929941458No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATT1979491.0918495871000589No Hit
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTG1776140.9796855380082234No Hit
TAGCTTATCAGACTGATGTTGACCTGGAATTCTCGGG1739210.9593156420998808No Hit
CGCTACCTCAGATCAGACGTGGCGACCCGCTGAATGG1736420.957776730386253No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGT1648740.909414085565146No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTG1606500.8861152931695763No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTT1498900.826765149661922No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGG1417080.7816347710206929No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGA1318570.7272985011606649No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGG1268220.6995263847516464No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTG1250950.6900005764024161No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGT1195080.6591837314417038No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTT1183920.6530280845871925No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAA1151970.6354050633504865No Hit
TAGCTTATCAGACTGATGTTGACATGGAATTCTCGGG1050110.5792209962715865No Hit
AGCTACATCTGGCTACTGGGTCTCTGGAATTCTCGGG1019310.5622322934831501No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAAT967640.5337320898117701No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGG935250.5158663728209438No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTG921760.5084255416321124No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATG815940.4500572127661276No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGG800170.44135877630594444No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTG750270.41383487146364023No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGG704170.3884069754069222No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTG642480.3543799275167067No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTG631360.34824634391257No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGG547820.3021672455052333No Hit
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGG528780.2916651383269272No Hit
TTTGGCAATGGTAGAACTCACACTGTGGAATTCTCGG503750.2778590594050258No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA457360.25227120478309206RNA PCR Primer, Index 1 (100% over 22bp)
AGCTACATCTGGCTACTGGGTCTCTTGGAATTCTCGG418550.23086433610714358No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGG413650.22816158793625596No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGA400690.22101309481488796No Hit
TTACTTGATGACAATAAAATATCTGATATGGAATTCT386370.21311445118078381No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCATGG384310.21197819378649227No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCG384240.21193958309833671No Hit
TGAGCATGTAGACAAAGGTAACACTGAAGTGGAATTC382150.2107867782662643No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGG380010.20960639437122355No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGT379680.20942437255563315No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGT376080.20743868002191984No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGC368700.2033680103278075No Hit
ATTACTTGATGACAATAAAATATCTGATATGGAATTC364720.2011727169155355No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGG362890.20016332321089791No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGT359750.1984313580564924No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGT355650.19616987489309667No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGT348350.1921433316997335No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTG344010.1897494690340902No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT337490.1861531592230316No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTG323790.17859649596973362No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGA320680.17688107825310906No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTTG318490.1756731152951001No Hit
TCCCTGAGACCCTTTAACCTGTGATGGAATTCTCGGG314140.17327373681686317No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAAT290890.16044947253663122No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTC287860.1587781813207558No Hit
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGG275930.15219781689653356No Hit
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCG271990.1500245867346362No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAA271160.1495667742893634No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGG269940.1488938451529383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGA265820.14662133036435526No Hit
CACCCGTAGAACCGACCTTGCGTGGAATTCTCGGGTG258940.14282645129992533No Hit
AACATTCAACGCTGTCGGTGAGTTTTGGAATTCTCGG255570.14096762245586592No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTC244430.13482300722654186No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAA241400.1331517160106665No Hit
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGT236520.13045999946496617No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTG233970.12905346725358588No Hit
TAGCACCATCTGAAATCGGTTATGGAATTCTCGGGTG232500.12824264280231962No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTG227330.12539096769140354No Hit
AGGAGATTTCAACTTAACTTGACCGCTCTGACCTGGA226190.12476216505572764No Hit
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTG221590.12222489126264947No Hit
TTTGGCAATGGTAGAACTCACACTGGAATTCTCGGGT218320.12042122054452653No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG211110.11644431966450622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAT207370.1143814057544818No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACTGGAAT199740.11017284074552826No Hit
AAGTGTTTGTGGGTTTAAGTCCCATTGGTCTAGCCAT196580.10842984396593545No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTG188950.10422127895698191No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGC186400.10281474674560162No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTG184880.10197634323136712No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)